Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Please note that all translations are automatically generated. Click here for the English version.
37.0K Views
•
10:18 min
•
February 9th, 2015
DOI :
February 9th, 2015
•Rozdziały w tym wideo
0:05
Title
1:30
In Silico Design: Final Plasmid and Overlapping Primers
3:58
Amplification of the DNA Fragments
5:44
DNA Purification Using Magnetic Beads
6:58
Assembly Cloning Reaction and Transformation of the Products in E. coli
8:21
Results: Cloning of CstF-54 and CstF-64 Mutants Under the Transcriptional Regulation of Human Elongation Factor 1α Promoter
9:47
Conclusion
Powiązane Filmy
12:02
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
20.6K Views
10:09
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
13.2K Views
10:46
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
9.2K Views
10:00
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
9.7K Views
10:38
Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
9.5K Views
09:27
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
7.5K Views
13:17
Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
14.3K Views
08:31
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
13.2K Views
Construction of Adenoviral Vectors using DNA Assembly Technology
1.3K Views
09:59
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
2.6K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone