The In Ovo Chick Chorioallantoic Membrane (CAM) Assay as an Efficient Xenograft Model of Hepatocellular Carcinoma

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35.1K Views

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08:17 min

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October 9th, 2015

DOI :

10.3791/52411-v

October 9th, 2015

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Michael Li¹^,², Ravi R. Pathak⁵, Esther Lopez-Rivera³, Scott L. Friedman¹, Julio A. Aguirre-Ghiso⁴, Andrew G. Sikora⁵

¹Department of Medicine, Division of Liver Diseases, Icahn School of Medicine at Mount Sinai, ²Department of Otolaryngology, Icahn School of Medicine at Mount Sinai, ³Division of Nephrology, Columbia University College of Physicians and Surgeons, ⁴Departments of Medicine, Hematology and Medical Oncology, Icahn School of Medicine at Mount Sinai, ⁵Bobby R. Alford Department of Otolaryngology - Head and Neck Surgery, Baylor College of Medicine

Podsumowanie

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Keywords Chick Chorioallantoic Membrane CAM Assay

Xenograft Model

Hepatocellular Carcinoma HCC

Angiogenesis

Tumor Growth

Anti tumor Therapies

Extracellular Matrix Proteins

Tumor Invasion

Metastasis

Rozdziały w tym wideo

0:05

Title

1:44

Preparing the Egg for Inoculation: "Dropping" the CAM and Opening the Egg

3:57

Inoculation of the CAM with Tumor Cells

5:32

Harvesting Tumors from the CAM

6:24

Tumor Cell Dissociation

7:20

Results: The CAM Supports Growth of Three-Dimensional, Vascularized Tumors

7:44

Conclusion

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