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Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule

11.7K Views

13:30 min

November 25th, 2015

DOI :

10.3791/53144-v

November 25th, 2015


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Ramsay Assay

Rozdziały w tym wideo

0:05

Title

0:53

Preparing Tubule Transfer Rods

1:33

Preparing the Ion-Specific Electrodes

3:38

Preparing the Reference Electrodes and Calibrating the Electrodes

7:16

Tubule Dissection

9:41

Making Measurements

11:06

Results: Measurement K+ and Na+ Ion Fluxes to Investigate the Role of the Sodium-potassium-2-chloride Cotransporter (NKCC)

12:07

Conclusion

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