Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
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11.4K Views
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10:18 min
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October 10th, 2015
DOI :
October 10th, 2015
•Laura Gouder1,2,3, Jean-Yves Tinevez4, Hany Goubran-Botros1,2,3, Alexandra Benchoua5, Thomas Bourgeron1,2,3, Isabelle Cloëz-Tayarani1,2,3
1Human Genetics and Cognitive Functions, Institut Pasteur, 2CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, 3Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, 5Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry
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Title
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Neuronal Culture and Lentiviral Transduction
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Immunofluorescence Dendritic Spine Imaging
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3D Quantification of Dendritic Spines
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Results: Spine Quantification of Cultured Dendrites
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Conclusion
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