CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

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11.3K Views

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11:38 min

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February 29th, 2016

DOI :

10.3791/53597-v

February 29th, 2016

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Rafael D. Melani*¹^,², Henrique S. Seckler*¹, Owen S. Skinner¹, Luis H. F. Do Vale¹^,³, Adam D. Catherman¹, Pierre C. Havugimana¹, Marcelo Valle de Sousa³, Gilberto B. Domont², Neil L. Kelleher¹, Philip D. Compton¹

¹Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, ²Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, ³Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

Podsumowanie

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Keywords CN GELFREE

Native Gel Electrophoresis

Macromolecular Complexes

Protein Complexes

Native Separations

Gradient Gel Electrophoresis

Gradient Mixer

Casting System

Gel Casting

Non covalent Interactions

Rozdziały w tym wideo

0:05

Title

0:38

Prepararing the Gradient Tube Gels

4:44

Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis (CN-GELFrEE) Setup

6:59

CN-GELFrEE: Sample Loading to Analysis

9:13

Results: Non-covalent Protein Assemblies from Mouse Heart

11:00

Conclusion

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