A Quantitative Glycomics and Proteomics Combined Purification Strategy

14.8K Views

11:38 min

March 8th, 2016

DOI :

10.3791/53735-v

March 8th, 2016


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Glycomics

Rozdziały w tym wideo

0:05

Title

1:10

Protein and Glycoprotein Denaturation and Alkylation

2:15

N-linked Glycan Enzymatic Digestion

3:29

Elution of N-glycans and Protein Filter Aided Sample Preparation (FASP) Digestion

6:37

Derivatization of N-linked Glycans with Hydrophobic Hydrazide Tags

8:19

Ultra-high Pressure Liquid Chromatography and Mass Spectrometry Analysis

9:27

Results: Analysis of the Glycomics and Proteomics Combined Purification Strategy

10:44

Conclusion

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