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Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

11.8K Views

11:11 min

February 17th, 2016

DOI :

10.3791/53764-v

February 17th, 2016


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Oligodendrocyte Progenitor Cells

Rozdziały w tym wideo

0:05

Title

0:50

Rat Brain Dissection

4:20

Enzymatic and Mechanical Tissue Dissociation

5:26

Anti-A2B5 Bead Application, Column Purification, and Plating

6:29

Induction of OPC Differentiation and Fixation with 4% Paraformaldehyde

7:38

Immunocytochemistry and Qualification of Myelin Basic Protein

9:25

Results: OPC Culture and Differentiation Procedure

10:29

Conclusion

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