TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

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5.6K Views

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09:18 min

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June 2nd, 2016

DOI :

10.3791/53879-v

June 2nd, 2016

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Arisa Haino*¹, Tatsuya Ishikawa*¹, Mineaki Seki¹, Masayuki Nashimoto¹

¹Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences

Transkrypcja

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TRUE Gene Silencing

Small Guide RNA sgRNA

Heptamer type SgRNA Library

TRNase ZL

RNA directed Gene Silencing

Cancer Therapeutic Agents

Rozdziały w tym wideo

0:05

Title

0:41

Construction of a Heptamer-type sgRNA Library

4:26

Screening of the sgRNA Library for Cell Viability

5:44

Evaluation of Effective sgRNAs by Flow Cytometry

7:15

Effects of Heptamer-type sgRNAs on Leukemia and Myeloma Cell Lines

8:41

Conclusion

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