Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

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9.3K Views

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09:31 min

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October 16th, 2016

DOI :

10.3791/54072-v

October 16th, 2016

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Yeri Alice Rim¹^,³, Narae Park¹, Yoojun Nam¹, Ji Hyeon Ju²^,³

¹CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, Republic of Korea, ²Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, Republic of Korea, ³College of Medicine, The Catholic University of Korea, Republic of Korea

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Keywords Induced pluripotent Stem Cells

IPSCs

Fibroblast like Synoviocytes

Rozdziały w tym wideo

0:05

Title

0:59

Fibroblast-like Synoviocyte (FLS) Isolation

2:51

FLS Maintenance and Expansion

3:51

FLS Reprogramming via Yamanaka Factors-encoded Lentivirus

6:08

Colony Picking

7:09

Results: Representative FLS-iPSC Analyses

8:39

Conclusion

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