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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

28.8K Views

11:02 min

May 17th, 2016

DOI :

10.3791/54112-v

May 17th, 2016


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Histone

Rozdziały w tym wideo

0:05

Title

1:07

Isolation of Nuclei from Intact Cells

3:18

Extraction and Purification of Histones from Nuclei

5:53

Chemical Derivatization of Histones Using Propionic Anhydride for Bottom-up Analysis

7:43

Sample Desalting with Stage-tips

8:59

Results: Characterization of Histone Post-translational Modifications

10:10

Conclusion

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