Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

1:00

Methodologies

2:20

Introduction

3:12

Tissues to be Harvested

3:53

Preparation of the Placenta and the Placental Anatomy

4:40

Dissection of the Decidua (D) Tissue

5:40

Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue

6:36

Mincing and Enzymatic Digestion of the Placental Tissue

7:25

Collection of Mononuclear Cells and Plating into Flasks

9:33

Post Isolation - Cell Growth and Expansion

9:57

Representative Results: Culture MSC and Assay Cells for MSC Characteristics

12:07

Conclusion