Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
36.1K Views
•
12:40 min
•
June 6th, 2016
DOI :
June 6th, 2016
•Chapters in this video
0:00
Title
1:00
Methodologies
2:20
Introduction
3:12
Tissues to be Harvested
3:53
Preparation of the Placenta and the Placental Anatomy
4:40
Dissection of the Decidua (D) Tissue
5:40
Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue
6:36
Mincing and Enzymatic Digestion of the Placental Tissue
7:25
Collection of Mononuclear Cells and Plating into Flasks
9:33
Post Isolation - Cell Growth and Expansion
9:57
Representative Results: Culture MSC and Assay Cells for MSC Characteristics
12:07
Conclusion
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