Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

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12.7K Views

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10:16 min

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September 15th, 2016

DOI :

10.3791/54543-v

September 15th, 2016

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Andrzej Chruscinski¹, Flora Y. Y. Huang¹, Antigona Ulndreaj², Conan Chua¹, Michael Fehlings³, Vivek Rao⁴, Heather J. Ross¹, Gary A. Levy¹

¹Multi-Organ Transplant Program, University Health Network, ²Institute of Medical Science, University of Toronto, ³Divison of Neurosurgery, University Health Network, ⁴Division of Cardiac Surgery, University Health Network

Podsumowanie

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Antigen Microarrays

Microarray Printing

Antigen Dilution

Positive Controls

Negative Controls

Humidity Control

Parallel Screening

Rozdziały w tym wideo

0:05

Title

0:35

Dilution Antigens and Generating Antigen Microarrays

3:21

Probe Antigen Microarrays with Diluted Sera

6:02

Scanning Antigen Microarrays and Exporting Data

7:40

Results: Detection of IgG and IgM Autoantibodies using Two-color Microarrays

9:29

Conclusion

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