Dilution Antigens and Generating Antigen Microarrays
3:21
Probe Antigen Microarrays with Diluted Sera
6:02
Scanning Antigen Microarrays and Exporting Data
7:40
Results: Detection of IgG and IgM Autoantibodies using Two-color Microarrays
9:29
Conclusion
Transkript
The overall goal of this technique is to rapidly screen for autoantibodies in a multiplex fashion. This method can help identify key questions in the field of autoimmunity, such as which autoantibodies are correlated with different disease states.
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.