Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.
13.2K Views
•
11:32 min
•
September 28th, 2016
DOI :
September 28th, 2016
•Rozdziały w tym wideo
0:05
Title
1:08
Liposome Mediated Transient Transfection
2:35
Preparing Cell Samples for Staining
4:51
Extracellular Staining of Cell Samples
6:10
Intracellular Staining of Cell Samples
7:51
Data Analysis
8:55
Results: Simultaneaous Mutations of N-glycosylation Sites Disrupted Cell Surface Expression of CaVα2δ1
10:25
Conclusion
Powiązane Filmy
11:54
Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
8.9K Views
10:46
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
3.5K Views
08:26
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
2.3K Views
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
32.4K Views
07:20
Measuring Erythrocyte Complement Receptor 1 Using Flow Cytometry
7.1K Views
11:11
Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
18.3K Views
10:01
Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
3.8K Views
06:56
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
13.6K Views
05:58
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
10.9K Views
07:29
Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry
1.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone