We thank the Engineering and Physical Sciences Research Council (EPSRC) for financial support (HLB). We also thank Srid&#233;vi Maalika Ramanoudjame for her assistance during the filming of this procedure.

1. Solid-phase Synthesis of Peptoids
NOTE: Peptoids are synthesized manually using the submonomer procedure of solid-phase peptoid synthesis. This method allows high coupling efficiency and good final product yields. Synthesis on solid-phase also allows excess reactants to be removed easily at the end of each step and the method has been modified here to allow different functionalized cationic monomers (i.e., arginine-type and lysine-type residues) to be included within the same sequence.1,2
<ol>
	<li>Synthesis of a linear peptoid 
	Caution: Carry out safety assessments before starting synthesis. Carry out all reactions in a fume hood and wear adequate personal protective equipment as appropriate (i.e., disposable nitrile gloves, safety glasses and a lab coat). Take particular care when using the following reagents and solvents. Dimethylformamide (DMF) is a suspected teratogen and dichloromethane (DCM) is a carcinogen. N,N&#39;-diisopropylcarbodiimide (DIC) and piperidine are hazardous to eyes, skin, via respiratory inhalation and may cause skin sensitization. Hydrazine is a suspected carcinogen, fatal if inhaled and causes severe burns to skin or eyes. Bromoacetic acid is also hazardous to skin, eyes and the respiratory tract and may cause burns upon contact. Trifluoroacetic acid (TFA) is a volatile liquid and can cause severe burns so handle with care. Heavy duty gloves are recommended.
	<ol>
		<li>Add 0.12 g Fmoc-protected Rink Amide resin (0.1 mmol, typical loading 0.7 mmol/g) to a capped 20 ml polypropylene reaction vessel with two frits. Add 5 ml dimethylformamide (DMF) to swell the resin and leave the vessel to stand for at least 60 minutes at room temperature. Drain the DMF using a solid-phase extraction vacuum platform.</li>
		<li>To deprotect the Fmoc group on the swollen resin, add 2 ml piperidine solution (20% in DMF v/v). Place the vessel on a shaker platform at room temperature (450 rpm) and shake for 5 min. Remove the solution via vacuum station.
		<ol>
			<li>Repeat Fmoc deprotection with 2 ml piperidine solution and shake for 15 min at room temperature. Drain the solution as before.</li>
		</ol>
		</li>
		<li>Wash the resin by adding 2 ml DMF and mixing the resin for 30 sec. Drain the DMF and repeat three more times.</li>
		<li>For the acetylation, add 1 ml of bromoacetic acid solution (0.6 M in DMF) and 0.2 ml N,N&#39;-diisopropylcarbodiimide solution (DIC, 50% in DMF v/v). Leave reaction vessel to shake for 20 min at room temperature. Drain the solution and wash the resin with 2 ml DMF three times.</li>
		<li>For the displacement, add 1 ml of amine solution (1.5 M in DMF). Shake the resin for 60 min at room temperature. Drain the solution and wash the resin with 2 ml DMF three times.
		<ol>
			<li>To add an arginine-type monomer, follow step 1.7. Depending upon the desired peptoid sequence, different amines will be added.</li>
		</ol>
		</li>
		<li>Repeat steps 1.1.4 and 1.1.5.</li>
		<li>To include a guanidine functionalized monomer (i.e., NArg), add 1 ml of unprotected diamine solution (1.5 M in DMF) to the resin and shake for 60 min at room temperature.
		<ol>
			<li>Drain the solution and wash the resin with 2 ml DMF three times.</li>
			<li>Add 2-acetyldimedone (0.2 g, 1 mmol in 0.5 ml DMF, 10 equivalents) to add the Dde group to the free primary amine and shake for 60 min at room temperature. Drain the solution and wash the resin with 2 ml DMF three times.</li>
		</ol>
		</li>
		<li>Continue submonomer synthesis as in 1.1.4 to 1.1.7 until the desired sequence is made. Add 2 ml DMF to wash the resin and repeat three times.</li>
		<li>To deprotect the Dde group on resin, add 4 ml 2% hydrazine solution (in DMF v/v) and shake for 3 min at room temperature. Drain the solution and repeat three times.</li>
		<li>Drain the solution and wash the resin with 2 ml DMF three times.</li>
		<li>Add pyrazole-1-carboxamidine (6 equivalents per free amine, i.e., per NArg monomers, in the minimum volume of DMF) and N,N-diisopropylethylamine or DIPEA (6 equivalents per free amine) and shake at room temperature for 60 min.</li>
		<li>Drain the solution and wash the resin with 2 ml dichloromethane three times. Leave the resin to dry in air for 10 min then the resin can be stored until cleavage (section 2).</li>
		<li>To pause the synthesis, wash the resin with 2 ml DMF three times. Add 2 ml DMF, stopper the synthesis vessel and leave at room temperature in a fume hood. 
		NOTE: The synthesis may be paused after any displacement step (except the second displacement step as diketopiperazines may be formed).</li>
	</ol>
	</li>
	<li>Side-chain deprotection and cleavage from resin
	<ol>
		<li>Undertake a test cleave to check the progress of synthesis (purity and mass) at any point during synthesis after the displacement steps, addition or removal of Dde-protecting group or after the final sequence has been made.
		<ol>
			<li>Transfer approximately 10 resin beads from the reaction vessel into a new 8 ml polypropylene fritted cartridge.</li>
			<li>Add 1 ml of trifluoroacetic acid cleavage cocktail (containing 95% TFA, 2.5% H2O, 2.5% triisopropylsilane) and shake for 90 min at room temperature.</li>
			<li>Filter the TFA cleavage cocktail from the resin using the fritted reaction vessel into a 10 ml round bottomed flask.</li>
			<li>Evaporate the cleavage cocktail using a rotary evaporator and redissolve the resulting oil in 1 ml acetonitrile/water for submission to LC-MS or analytical HPLC.</li>
		</ol>
		</li>
		<li>For the final cleavage: in the same fritted polypropylene reaction cartridge used for synthesis, add 4 ml of the TFA cleavage cocktail (95% TFA, 2.5% H2O, 2.5% triisopropylsilane) and cover the vessel. Shake for 90 min at room temperature.</li>
		<li>Filter the TFA cleavage cocktail from the resin using the fritted reaction vessel into a 50 ml round bottomed flask.</li>
		<li>Evaporate the cleavage cocktail using a rotary evaporator. After the TFA has been removed, the product should be obtained as an oil. To aid TFA removal from this crude oil, add 2 ml anhydrous diethyl ether and the peptoid should precipitate.
		<ol>
			<li>Either remove the diethyl ether via pipette and discard or evaporate using a rotary evaporator. Repeat diethyl ether precipitation three times.</li>
		</ol>
		</li>
		<li>Dissolve the crude peptoid in 10 ml acetonitrile/acidified water solution (50% acetonitrile, 0.1% TFA in water v/v). Transfer to a pre-weighed container, freeze at 20 &#176;C and lyophilize to a dry powder.</li>
	</ol>
	</li>
</ol>
2. Characterization and Purification
NOTE: The peptoid synthesis can be monitored and the final peptoid assessed via analytical reverse-phase HPLC using a C18 column and electrospray liquid-chromatography mass spectrometry (LC-MS). All HPLC solvents of solvents for LC-MS should be freshly prepared.
<ol>
	<li>Analytical HPLC
	<ol>
		<li>Weigh 1 mg of peptoid in a small glass vial. Add the minimum volume of acetonitrile to dissolve and dilute to 1 ml with water. Ensure that the peptoid has completely dissolved.</li>
		<li>Inject 10 &#181;l to analytical HPLC (suggested gradient 0 - 100% solvent B over 30 min, where solvent A = 95% water, 5% acetonitrile, 0.05% TFA and solvent B = 95% acetonitrile, 5% water, 0.03% TFA), as per manufacturer&#39;s instructions.</li>
		<li>Visualize UV spectrum at 220 nm.</li>
	</ol>
	</li>
	<li>ESI LC-MS
	<ol>
		<li>Make 1 mg/ml peptoid solution as in step 2.1.1.</li>
		<li>Inject 1 &#181;l to electrospray LC-MS to determine if the molecular weight of the target peptoid is present, using manufacturer&#39;s instructions.</li>
		<li>Check the target mass of the peptoid sequence using a peptoid calculator, as per instructions on the calculator. 26 This web utility also allows the assignment of any deletion/addition products seen in the mass spectrum.26</li>
	</ol>
	</li>
	<li>Preparative reverse phase HPLC
	<ol>
		<li>Dissolve crude peptoids into 2 ml acidified water/acetonitrile (95% water, 5% MeCN, 0.1% TFA) and purify by preparative RP-HPLC using manufacturer&#39;s protocol. Determine the gradient by the elution time obtained from analytical HPLC and the amount injected will depend upon the column dimensions.</li>
		<li>Visualize using a detector set at 220 nm.</li>
		<li>Collect fractions in 15 ml centrifuge tubes, freeze at 20 &#176;C and lyophilize.</li>
		<li>Re-analyze fractions using LC-MS and analytical HPLC according to manufacturer&#39;s protocol. Recombine purified fractions.</li>
	</ol>
	</li>
</ol>
3. Biological Testing against Leishmania mexicana Parasites
Caution: Safety assessments must be carried out before starting synthesis. Leishmania mexicana is classified a hazard group 2 pathogen in the U.K. and adequate control measures must be in place prior to starting testing. All work must be carried out in a class 2 microbiological safety cabinet and adequate personal protective equipment worn as appropriate (i.e., nitrile gloves, safety glasses and a lab coat).
<ol>
	<li>Subculture of parasites
	<ol>
		<li>Defrost 1 ml -150 &#176;C frozen stock Leishmania mexicana M379 by placing vial in a 37 &#176;C water bath for 30 sec.</li>
		<li>Transfer the stock solution to 10 ml Schneider&#39;s Insect medium (at pH 7.0 supplemented with 15% heat-inactivated fetal bovine serum and 1% penicillin/streptomycin) in a 25 cm3 cell culture flask with a non-vented cap.</li>
		<li>Incubate at 26 &#176;C for 72 hr.</li>
		<li>Examine parasites under microscope (400X magnification) to check condition. They should be insect stage promastigotes, procyclic forms in log phase with many dividing cells.</li>
		<li>Maintain procyclic promastigotes by sub-culturing parasites to a concentration of 5 x 105 parasites/ml every three days. Count cells using a Neubauer improved hemocytometer.</li>
	</ol>
	</li>
	<li>Transformation of L. mexicana insect stage into mammalian stage axenic amastigote form 27
	<ol>
		<li>Day 0: Prepare 10 ml culture of log stage parasites at 5 x 105 parasites/ml in Schneider&#39;s Insect medium (at pH 7.0 supplemented with 15% heat-inactivated fetal bovine serum and 1% penicillin/streptomycin) in a 25 cm3 cell culture flask with a non-vented cap.</li>
		<li>Incubate at 26 &#176;C for 48 hr.</li>
		<li>Day 3: Transfer 10 ml culture to a 50 ml centrifuge tube and centrifuge at 447 x g for 5 min.</li>
		<li>Pour off old medium and add 10 ml Schneider&#39;s Insect medium (at pH 5.5 supplemented with 20% heat-inactivated fetal bovine serum and 1% penicillin/streptomycin). Gently resuspend the pellet of parasites in the new medium using a pipette.</li>
		<li>Count the number of parasites using a Neubauer improved hemocytometer. Dilute to a concentration of 5 x 105 parasites/ml with pH 5.5 medium and transfer to a 25 cm3 cell culture flask.</li>
		<li>Incubate at 26 &#176;C for approximately 6 days.</li>
		<li>Day 9: Examine parasites under a microscope (400X). They should be in the non-replicating, infectious metacyclic promastigote stage.</li>
		<li>Count the number of parasites using a Neubauer improved hemocytometer. Dilute to a concentration of 5 x 105 parasites/ml with pH 5.5 medium.</li>
		<li>Remove 10 ml of cell culture and transfer to cell culture flask. Incubate at 32 &#176;C for 5 days.</li>
		<li>Day 14: Check appearance of parasites under the microscope (400X). They should be in the pathogenic amastigote stage, lacking the flagellum characteristic of promastigotes, and ready for assay.</li>
	</ol>
	</li>
	<li>Cytotoxicity assay on L. mexicana axenic amastigotes. 
	NOTE: The biological testing of peptoids uses high-throughput assays carried out in 96 well plates. This protocol describes the testing of L. mexicana axenic amastigotes, but identical assays can also be carried out on the promastigote stage parasites in appropriate media. Compounds are incubated with parasites at concentrations from 3 - 100 &#181;M for 60 min and then incubated for 24 hr following a 10-fold dilution. Results are gained by measuring the fluorescence of wells after incubation with Resazurin-based cell viability solution (e.g., alamarBlue).
	<ol>
		<li>Prepare compound stock solutions. Weigh out 1 mg of the final purified peptoid product using an analytical balance. Add the appropriate volume of molecular biology grade dimethylsulphoxide (DMSO) to a concentration of 5 mM. Make 6 &#181;l aliquots and freeze at -20 &#176;C.</li>
		<li>Prepare compound solutions on 96 well plates (a recommended plate layout can be seen in the results section, Figure 6) in triplicate from 100 to 3 &#181;M. Add 2 &#181;l of 5 mM stock solution of each compound in the top row (i.e., A). Add 48 &#181;l fresh Schneider&#39;s Insect medium (at pH 5.5 and 20% fetal bovine serum (FBS), 1% penicillin/streptomycin(P/S)) to top row using a multi-channel pipette. Add 25 &#181;l Schneider&#39;s Insect medium to all other rows (B - F).
		<ol>
			<li>Carry out a serial dilution by pipetting 25 &#181;l solution from the top row. Add to the row below and mix. Carry out dilutions until the last row, where the last 25 &#181;l solution should be discarded.</li>
			<li>Use amphotericin B (5 mM stocks) as a positive control and DMSO (2% solution) as a negative control in triplicate.</li>
		</ol>
		</li>
		<li>Prepare parasite solution: transfer culture to a 50 ml centrifuge tube and centrifuge at 447 x g for 5 min. Pour off old medium and add 10 ml Schneider&#39;s Insect medium (at pH 5.5 and 20% FBS, 1% P/S).</li>
		<li>Gently dissolve the pellet of parasites in the new medium using a pipette and count using a Neubauer improved hemocytometer. Dilute culture to 8 x 106 parasites/ml.</li>
		<li>Add 25 &#181;l L. mexicana culture to each well. Incubate plates for 60 min at 32 &#176;C.</li>
		<li>Remove plate from the incubator and remove 40 &#181;l solution from each well.</li>
		<li>Add 90 &#181;l fresh medium and incubate for 24 hr at 32 &#176;C.</li>
		<li>Add 10 &#181;l Resazurin-based cell viability solution to each well. Incubate for 4 hr at 32 &#176;C.</li>
		<li>Measure the fluorescence using a plate reader (&#955;ex = 540 nm, &#955;em = 600 nm), as per manufacturer&#39;s instructions. Analyze data by removing average background (from medium only wells) and comparing the fluorescence of wells after normalization with respect to the DMSO controls.</li>
	</ol>
	</li>
</ol>

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<li>Foged, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cellular+uptake+and+membrane-destabilising+properties+of+alpha-peptide%2Fbeta-peptoid+chimeras%3A+lessons+for+the+design+of+new+cell-penetrating+peptides%5BTitle%5D)+AND+%22BBA+Biomembranes%22%5BJournal%5D)">Cellular uptake and membrane-destabilising properties of alpha-peptide/beta-peptoid chimeras: lessons for the design of new cell-penetrating peptides.</a> BBA Biomembranes. 1778, 2487-2495 (2008).</li>
<li>Vedel, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Antiplasmodial+and+prehemolytic+activities+of+alpha-peptide-beta-peptoid+chimeras%5BTitle%5D)+AND+%22Chem+Bio+Chem%22%5BJournal%5D)">Antiplasmodial and prehemolytic activities of alpha-peptide-beta-peptoid chimeras.</a> Chem Bio Chem. 8, 1781-1784 (2007).</li>
<li>Lear, S., Cobb, S. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pep-Calc.com%3A+a+set+of+web+utilities+for+the+calculation+of+peptide+and+peptoid+properties+and+automatic+mass+spectral+peak+assignment%5BTitle%5D)+AND+%22J+Computer-Aided+Mol+Des%22%5BJournal%5D)">Pep-Calc.com: a set of web utilities for the calculation of peptide and peptoid properties and automatic mass spectral peak assignment.</a> J Computer-Aided Mol Des. , 1-7 (2016).</li>
<li>Bates, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Complete+developmental+cycle+of+Leishmania+mexicana+in+axenic+culture%5BTitle%5D)+AND+%22Parasitol%22%5BJournal%5D)">Complete developmental cycle of Leishmania mexicana in axenic culture.</a> Parasitol. 108, 1-9 (1994).</li>
<li>Gossage, S. M., Rogers, M. E., Bates, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Two+separate+growth+phases+during+the+development+of+Leishmania+in+sand+flies%3A+implications+for+understanding+the+life+cycle%5BTitle%5D)+AND+%22Int+J+Parasitology%22%5BJournal%5D)">Two separate growth phases during the development of Leishmania in sand flies: implications for understanding the life cycle.</a> Int J Parasitology. 33, 1027-1034 (2003).</li>
<li>Seo, J., Lee, B. C., Zuckermann, R. N., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aSeo%2C+J+%22Comp+Biomaterials%22">Comp Biomaterials</a>. Ducheyne, P., et al. 2, Elsevier. 53-76 (2011).</li>
<li>Nash, I. A., Bycroft, B. W., Chan, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dde+-+A+selective+primary+amine+protecting+group%3A+A+facile+solid+phase+synthetic+approach+to+polyamine+conjugates%5BTitle%5D)+AND+%22Tetrahedron+Lett%22%5BJournal%5D)">Dde - A selective primary amine protecting group: A facile solid phase synthetic approach to polyamine conjugates.</a> Tetrahedron Lett. 37, 2625-2628 (1996).</li>
<li>Culf, A. S., Ouellette, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Solid-phase+synthesis+of+N-substituted+glycine+oligomers+%28alpha-peptoids%29+and+derivatives%5BTitle%5D)+AND+%22Molecules%22%5BJournal%5D)">Solid-phase synthesis of N-substituted glycine oligomers (alpha-peptoids) and derivatives.</a> Molecules. 15, 5282-5335 (2010).</li>
<li>Tran, H., Gael, S. L., Connolly, M. D., Zuckermann, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Solid-phase+Submonomer+Synthesis+of+Peptoid+Polymers+and+their+Self-Assembly+into+Highly-Ordered+Nanosheets%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets.</a> J Vis Exp. , e3373(2011).</li>
<li>Burkoth, T. S., Fafarman, A. T., Charych, D. H., Connolly, M. D., Zuckermann, R. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Incorporation+of+unprotected+heterocyclic+side+chains+into+peptoid+oligomers+via+solid-phase+submonomer+synthesis%5BTitle%5D)+AND+%22J.+Am.+Chem.+Soc%22%5BJournal%5D)">Incorporation of unprotected heterocyclic side chains into peptoid oligomers via solid-phase submonomer synthesis.</a> J. Am. Chem. Soc. 125, 8841-8845 (2003).</li>
<li>Nakayama, G. R., Caton, M. C., Nova, M. P., Parandoosh, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+the+Alamar+Blue+assay+for+cellular+growth+and+viability+in+vitro%5BTitle%5D)+AND+%22J+Immunol+Methods%22%5BJournal%5D)">Assessment of the Alamar Blue assay for cellular growth and viability in vitro.</a> J Immunol Methods. 204, 205-208 (1997).</li>
</ol>

The authors have nothing to disclose.

Peptoids are increasingly being studied within the chemical biology and medicinal chemistry fields in applications such as novel therapeutics3-5, cell delivery agents18,20 and diagnostic tools.29 Typically, these sequences are cationic to provide a degree of selectivity for the pathogen over mammalian cells, the ability to penetrate through cell membranes and also aid solubility in aqueous systems. There are numerous examples of cationic peptoids that contain solely lysine- or arginine-mimetic residues in the literature. However, to date, the synthesis of peptoids that contain both of these cationic residues in the same sequence has been hindered by lack of a suitable synthetic procedure. The protocol described here allows mixed cationic peptoids to be synthesized in an efficient manner and is highly desirable as it offers a route to modulate the biological and chemical properties of amphipathic peptoids.
Our method uses an adaptation to the commonly used submonomer peptoid synthesis and permits the addition of both lysine- and arginine-type monomers within the same sequence. It uses room temperature couplings and established protecting group chemistry so it is anticipated that this method will be useful for the majority of research groups. To add orthogonal protection for the arginine-type residue, an unprotected diamine is added under standard displacement conditions and then protected in a 60-minute coupling with Dde-OH. A variety of diamines can be used which allows side chains from 2 carbons to 6 carbons long to be installed, i.e., 1,2-diaminoethane to 1,6-diaminohexane respectively. The protecting Dde-OH dissolves well in DMF and is a very efficient and selective protecting group for primary amines. The Dde-protection group leaves secondary amines unaffected, e.g., the unprotected N terminus of peptoid chain.30 One limitation is that all of the synthesis was undertaken manually; however, it is anticipated that the coupling conditions developed make the method amenable for use with automated peptide/peptoid synthesizers.
The on resin deprotection of the Dde-groups is undertaken using a 2% hydrazine solution in DMF to leave free amines that can be guanidinylated on resin using pyrazole-1-carboxamidine. Six equivalents of the pyrazole-1-carboxamidine and six equivalents of DIPEA are used per free amine on the resin (i.e., six equivalents for each NArg type monomer to be installed). Again, this reaction is also efficient and the pyrazole-1-carboxamidine reagent has good solubility in DMF. Completion of the reaction is typically seen via LC-MS after 60 minutes at room temperature.
Due to the versatility of the submonomer method, a wide variety of primary amines can be used in the displacement step so conditions may need to be optimized to increase coupling efficiency and overall product yields or purity.31 For the sequences discussed above, no special conditions were necessary for successful couplings. However, longer displacement times or higher amine concentrations could be used for problematic displacements (i.e., for poorly nucleophilic or sterically bulky amines). Some amines may not be completely soluble in DMF, in which case it is recommended to dissolve these in N-methyl-2-pyrrolidone (NMP), or other appropriate solvents for solid-phase reactions instead as in a previous comprehensive method for submonomer synthesis of peptoids.32 To incorporate monomers that contain unprotected heteroatoms in the side chains, acetylation using chloroacetic acid has been shown to be effective by other groups.33 Additionally, other resins may be used with this method to yield peptoids with different C terminal functionalization. Wang resins and 2-chlorotrityl chloride resins are routinely used in the submonomer synthesis of peptoids. For example, this method has been successfully used with 2-chlorotrityl chloride resin in our group.2 Different solid supports will require a different loading procedure to Rink Amide discussed here (dependent on the specific resin used) so this should be checked with the literature prior to synthesis.
Similar to peptide synthesis, the conditions for final cleavage of the peptoid off resin can also be optimized for the specific sequence. In this protocol, a TFA cleavage cocktail was used (with triisopropylsilane and water as scavengers). The peptoids presented here contained only Boc protection which is a reasonably acid labile group. To ensure complete deprotection of sequences with a greater proportion of protected residues or less acid labile protecting groups, longer cleavage times may be necessary (i.e., cleavage times in excess of 2 hours are recommended for sequences that contain Pbf or tertiary-butyl ester protected groups). Alternative scavengers can also be used for specialized side chains (for example, ethanedithiol or 2-mercaptoethanol are often used in peptides that have sulphur containing side chains like cysteine or methionine).
The biological assay presented is a standard cytotoxicity test which can be altered to suit different cell lines. It is important to note that each 96-well plate should contain sufficient controls to allow confidence in the results obtained. In this case, amphotericin B is used as a positive control as it is a known drug used to treat the disease and DMSO is used as a negative control as this is the solvent used to make compound stocks for the assay. If other cell lines are being used, alternative, suitable controls should be obtained and validated before use. L. mexicana is incubated with the peptoid at concentrations between 100 and 2 &#181;M for one hour, and then the parasite/peptoid solution is diluted by a factor of ten for overnight incubation (i.e., wells initially with 100 &#181;M peptoid stock are diluted to 10 &#181;M).
The cell viability reagent is added to each well (10% of total well volume) at the end of the assay. A visible color change is seen between wells with viable parasites (pink), control wells with no viable parasites (blue) and a spectrum between with intermediate numbers of viable parasites. The fluorescence is proportional to the number of living cells and corresponds to the metabolic activity of the cells; resazurin dye (non-fluorescent) is converted to the fluorescent resorufin by reduction reactions in metabolically active cells.34 In this assay, the incubation time with the cell viability reagent has been optimized for L. mexicana. Incubation times with the viability reagent will vary for plates seeded at different cell concentrations or with different cell lines (for example this method can also be used with mammalian cells). Dependent on the exact plate reader used, considerations need to be made before fluorescence measurements are taken. Any air bubbles in wells should be removed to ensure accurate readings can be taken. Some plate readers read from the bottom of plates, in which case flat bottomed 96 well plates should be used. Other machines may read from the top of the plate, so the lid of the plate should be removed before measurement.
Finally, in the future, this protocol may be compatible with automated synthesizers that are capable of making many sequences in parallel. Additionally, the synthesis of cyclic peptoids is also possible using this method. This protocol should provide researchers with a practical synthetic procedure that can be used to access novel peptoid scaffolds with both lysine- and arginine-type monomers, which may be of use in many applications, including materials or medicinal fields.

Peptoids (or poly-N-substituted glycines) are a class of peptide-mimetics that offer similar properties to peptides and as such are increasingly being investigated for medicinal and materials applications. In peptides, the side chain of each amino acid is connected to the &#945;-carbon of the amide backbone; in peptoids the side chains are shifted onto the nitrogen atom of the backbone. Crucially, this gives peptoids greater resistance to proteolysis.
Peptoids are commonly synthesized using the submonomer method pioneered by Zuckermann et al., where peptoid monomers can be built by sequential haloacetylation of an amine functionality attached to a solid support and subsequent displacement of the halogen using a primary amine.1 Our group has recently developed an adaptation to this submonomer method to allow lysine- and arginine-type peptoid residues to be included within the same peptoid sequence for the first time.2 This manual solid-phase approach to peptoid synthesis uses commercially available reagents and common laboratory equipment, making it accessible for the majority of laboratories. Peptoids have been shown to have promising activities against a wide range of Gram negative bacteria, Gram positive bacterial and fungal species that are comparable to many known antimicrobial peptides.3-9
In our work, peptoids have been used as novel anti-infective compounds for treatment of the neglected tropical disease leishmaniasis.5,10 Leishmaniasis is endemic in more than 80 countries worldwide and it is estimated that over 12 million people are infected globally.11 The disease is caused by protozoan parasites that are transmitted by the bite of a sandfly. Leishmania species can cause cutaneous leishmaniasis, a condition that leads to scarring and damage to mucous membranes, or the life-threatening visceral leishmaniasis, which causes fatal organ damage. No vaccine is currently available for this disease and existing treatments rely on a small number of drugs that have severe side effects. In addition, resistance to existing drugs is an emerging and serious problem so new treatments are desperately needed to effectively treat leishmaniasis in the future.12-16
In these antimicrobial applications, peptoids are often designed to be amphipathic with a mixture of cationic and hydrophobic monomers.3,4 This can give peptoids a degree of selectivity towards bacterial cells, reduce toxicity to mammalian cells, and to improve their activity as molecular transporters.17-20 The majority of the anti-infective peptoids in the literature contain cationic side chains that are exclusively comprised of either amino functionalized lysine-type monomers or arginine-type residues. Peptide-peptoid chimeras, where the cationic chains are comprised of the amino acids lysine or arginine, have also been synthesized to examine the effect of cationic groups on activity and toxicity.21-25
Poly-lysine peptoids can be easily synthesized using commercially available Boc-protected amines. The poly-arginine peptoids reported can be made using a method which uses pyrazole-1-carboxamidine as a guanidinylation agent.18 However, this can only be undertaken after the peptoid has been cleaved from the resin and Boc protection on side chains removed, so every lysine-type residue within the sequence is transformed into an arginine residue. In an effort to fine tune the chemical and biological properties of the compounds, we developed a method that allows dual cationic functionality (e.g., NLys and NArg) to be included in any given peptoid sequence for the first time.2
Herein, we describe the synthesis, purification and characterization of two novel peptoids that contain both lysine- and arginine-type residues in the same sequence. The method uses orthogonal N-Boc and N-Dde protection on resin with pyrazole-1-carboxamidine as a guanidinylation reagent. The biological evaluation of these peptoids is also described in cytotoxicity assays against Leishmania mexicana, the causative agent of cutaneous leishmaniasis. This provides a practical method to access peptoids with dual cationic functionality and to assess their biological activity. It is expected that this method will aid the synthesis of amphipathic peptoids by the peptoid community in the future.

<table><tbody><tr><td>Polypropylene solid phase extraction cartridges with two frits</td><td>Crawford Scientific</td><td>12131017 and 12131015</td><td>20 ml and 6 ml cartridges used in this preparation</td></tr><tr><td>Trifluoroacetic acid</td><td>Tokyo Chemical Industry (Europe)</td><td>T0431-100g</td><td>&gt;98%; CAUTION: can cause severe burns and respiratory irritation</td></tr><tr><td>&lt;em&gt;N,N&#039;&lt;/em&gt;-diisopropylcarbodiimide</td><td>Sigma Aldrich</td><td>38370-100ML</td><td>&gt;98%; CAUTION: hazardous to eyes, skin and via respiratory inhalation, may also cause sensitisation</td></tr><tr><td>Dimethylformamide</td><td>Fischer Scientific</td><td>10346180</td><td>HPLC grade; CAUTION: suspected teratogen</td></tr><tr><td>Acetonitrile</td><td>Fischer Scientific</td><td>10407440</td><td>HPLC grade</td></tr><tr><td>Dichloromethane</td><td>Fischer Scientific</td><td>10354263</td><td>99.8%; CAUTION: suspected carcinogen</td></tr><tr><td>Bromacetic acid</td><td>Sigma Aldrich</td><td>17000-100G</td><td>&gt;99%; CAUTION: causes burns and hazardous to skin, eyes and respiratory tract</td></tr><tr><td>(&lt;em&gt;S&lt;/em&gt;)-(-)-alpha methylbenzylamine</td><td>Sigma Aldrich</td><td>115568-100G</td><td>98%; CAUTION: harmful if swallowed, toxic in contact with skin, causes severe skin burns and eye damage, used to synthesise the Nspe monomer</td></tr><tr><td>&lt;em&gt;N&lt;/em&gt;-Boc 1,4-diaminobutane</td><td>Tokyo Chemical Industry (Europe)</td><td>A1373-25g</td><td>&gt;98%; CAUTION: causes severe skin burns and eye damage, used to synthesise the NLys monomer</td></tr><tr><td>&lt;em&gt;N&lt;/em&gt;-Boc 1,2-diaminoethane</td><td>Tokyo Chemical Industry (Europe)</td><td>A1371-25g</td><td>&gt;97%; CAUTION: causes severe skin burns and eye damage, used to synthesise the Nae monomer</td></tr><tr><td>1,2-diaminobutane</td><td>Sigma Aldrich</td><td>D13208-100G</td><td>99%; CAUTION: flammable liquid and vapour, harmful if swallowed or inhaled, toxic in contact with skin, causes severe skin burns and eye damage, used in the synthesis of the NhArg monomer</td></tr><tr><td>1,4-diaminoethane</td><td>Sigma Aldrich</td><td>03550-250ML</td><td>&gt;99.5%; CAUTION: flammable liquid and vapour, harmful if swallowed or inhaled, toxic in contact with skin, causes severe skin burns and eye damage, used in the synthesis of the NnArg monomer&lt;br /&gt;&amp;nbsp;&lt;br /&gt;&amp;nbsp;</td></tr><tr><td>1H-pyrazole-1-carboxamidine HCl</td><td>Sigma Aldrich</td><td>402516-10G</td><td>as HCl salt; CAUTION: harmful if swallowed and may cause an allergic skin reaction, causes serious eye damage</td></tr><tr><td>Hydrazine monohydrate</td><td>Sigma Aldrich</td><td>207942-5G</td><td>reagent grade; CAUTION: suspected carcinogen, fatal if inhaled and causes severe burns to skin and eyes</td></tr><tr><td>2-acetyl dimedone</td><td>Novabiochem</td><td>8510150005</td><td>Dde-OH</td></tr><tr><td>Rink Amide resin</td><td>Novabiochem</td><td>8551190005</td><td>100-200 mesh, high loading</td></tr><tr><td>Piperidine</td><td>Sigma Aldrich</td><td>411027-1L</td><td>&gt;99.5%, a controlled substance, so adequate permission must be obtained before purchase; CAUTION: highly flammable liquid and vapour, harmful if swallowed and toxic in contact with skin or if inhaled, causes severe skin burns and eye damage, harmful to aquatic life with long lasting effects</td></tr><tr><td>Triisopropylsilane</td><td>Sigma Aldrich</td><td>233781-50G</td><td>98%; CAUTION: flammable liquid and vapour, causes skin irritation and serious eye irritation</td></tr><tr><td>alamarBlue</td><td>ThermoFischer&amp;nbsp;</td><td>DAL1025</td><td>Not classified as hazardous</td></tr><tr><td>Schneider&#039;s Insect Medium</td><td>Sigma Aldrich</td><td>S9895-1L</td><td>Powdered, medium must be made prior to use following manafacturers instructions; allow to warm to room temperature before use in biological assays</td></tr><tr><td>96 well plates</td><td>VWR</td><td>734-1793</td><td>Flat bottom (to allow fluorescence measurement from the bottom), tissue-culture treated</td></tr><tr><td>Solvent reservoirs</td><td>VWR</td><td>613-1182</td><td>Used with multi channel pipette</td></tr><tr><td>Multi channel pipette</td><td>Eppendorf</td><td>3122000043</td><td></td></tr><tr><td>Pipette tips</td><td>Starlab Group</td><td>S1111-3810, S1113-1810, S1111-6810</td><td>Volume of tip dependent on pipette used. 10&amp;nbsp;&amp;micro;l, 10 - 200&amp;nbsp;&amp;micro;l and 1,000&amp;nbsp;&amp;micro;l recommended for assays</td></tr><tr><td>25 cm&lt;sup&gt;3&lt;/sup&gt; cell culture flasks</td><td>VWR</td><td>734-2312</td><td></td></tr><tr><td>50 ml centrifuge tubes</td><td>VWR</td><td>525-0791</td><td></td></tr><tr><td>dimethylsulphoxide (molecular biology grade)</td><td>Sigma Aldrich</td><td>D8418-50ML</td><td>Not classified as hazardous</td></tr><tr><td>Heat Inactivated Fetal Bovine Serum</td><td>ThermoFischer&amp;nbsp;</td><td>10082139-100mL</td><td>Gibco</td></tr><tr><td>Penicillin/Streptomycin</td><td>ThermoFischer&amp;nbsp;</td><td>15140148-20mL</td><td>Abbreviation: P/S</td></tr><tr><td>Amphotericin B</td><td>Sigma Aldrich</td><td>46006-100mg</td><td>Amphotericin B trihydrate, VetranalTM analytical standard</td></tr></tbody></table>

an efficient method for the synthesis of peptoids with mixed lysine-type/arginine-type monomers and evaluation of their anti-leishmanial activity

This protocol describes the manual solid-phase synthesis of linear peptoids that contain two differently functionalized cationic monomers. In this procedure amino functionalized 'lysine' and guanido functionalized 'arginine' peptoid monomers can be included within the same peptoid sequence. This procedure uses on-resin (N-(1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl) or Dde protection, orthogonal conditions to the Boc protection of lysine monomers. Subsequent deprotection allows an efficient on-resin guanidinylation reaction to form the arginine residues. The procedure is compatible with the commonly used submonomer method of peptoid synthesis, allowing simple peptoids to be made using common laboratory equipment and commercially available reagents. The representative synthesis, purification and characterization of two mixed peptoids is described. The evaluation of these compounds as potential anti-infectives in screening assays against Leishmania mexicana is also described. The protozoan parasite L. mexicana is a causative agent of cutaneous leishmaniasis, a neglected tropical disease that affects up to 12 million people worldwide.

As a representative result, the synthesis and characterization of two 12 residue peptoids containing two lysine-type monomers and two-arginine type monomers each will be described. The subsequent results from cytotoxicity assay are also shown.
Two peptoids [(NnArgNspeNspe)(NaeNspeNspe)]2 (a) and [(NhArgNspeNspe)(NLysNspeNspe)]2 (b) were synthesized using 120 mg Rink Amide resin each (loading = 0.79 mmol/g). All acetylation and displacement steps were carried out as described above, with all reagents purchased commercially. For these residues, the following amines were used in the displacement step: Nspe (S)-(-)-&#945;-methylbenzylamine, Nae N-(tert-butoxycarbonyl)-1,2-diaminoethane, NLys N-(tert-butoxycarbonyl)-1,4-diaminobutane. For the arginine derivative residues, the following unprotected diamines were coupled: NhArg 1,4-diaminobutane or NnArg 1,2-diaminoethane, followed by on-resin protection with 2-acetyldimedone (Dde-OH). After the entire sequence had been synthesized, hydrazine deprotection of the Dde yields free amines to guanidinylate.
<img alt="Figure 1" src="/files/ftp_upload/54750/54750fig1.jpg" /> 
Figure 1. Peptoid structures (a) [(NnArgNspeNspe)(NaeNspeNspe)]2 and (b)&#160;[(NhArgNspeNspe)(NLysNspeNspe)]2. <a href="https://www.jove.com/files/ftp_upload/54750/54750fig1large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" src="/files/ftp_upload/54750/54750fig2.jpg" /> 
Figure 2. The method used to synthesize mixed arginine/lysine peptoids.&#160;i. Standard displacement step in the submonomer method with diamine; ii. Addition of Dde-OH, 90 minutes to protect free amine; iii. Further additions to extend the peptoid chain using the submonomer method; iv. Deprotection of Dde using 2% hydrazine in DMF; v. Guanidinylation of free amine on resin with pyrazole-1-carboxamidine and DIPEA in DMF; vi. Acidic cleavage from the resin and deprotection of Boc groups. <a href="https://www.jove.com/files/ftp_upload/54750/54750fig2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Following cleavage from the resin and lyophilization, the crude products were obtained as white powders: (a) 154 mg, (b) 163 mg. Products were purified via RP-HPLC as described with maximum 50 mg injections using a LC pump with a UV-vis detector (&#955; = 250 nm) on an analytical column, 250 mm x 10 mm, 5 &#181;m; flow rate = 2 ml/min. Fractions corresponding to the target mass were combined and obtained as white powders: (a) 54 mg (b) 65 mg, final yields of approximately 30% for fractions &#62;90% pure.
The final compound identities after purification were confirmed by LC-MS (see Figure 3) using a triple quadrupole mass spectrometer equipped with an UPLC and a photodiode array detector. Accurate mass spectrometry was undertaken using the same spectrometer on the [M+2H]2+ ions. The following calculated and observed masses were found in close agreement (Figure 4): (a) calculated = 896.0026 amu, observed = 896.0038 amu; (b) calculated = 952.0691 amu, observed = 952.0730 amu.
<img alt="Figure 3" src="/files/ftp_upload/54750/54750fig3.jpg" /> 
Figure 3. LC-MS for purified peptoids. (a) m/z = 1,792 and (b) m/z = 1,903. Where the top is TIC, middle LC-MS spectrum, bottom UV chromatogram at 220 nm. <a href="https://www.jove.com/files/ftp_upload/54750/54750fig3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" src="/files/ftp_upload/54750/54750fig4.jpg" /> 
Figure 4. Accurate mass spectrometry data for peptoids (a) and (b). <a href="https://www.jove.com/files/ftp_upload/54750/54750fig4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Product purity was assessed using an analytical RP-HPLC (LC pump with a UV-vis detector on an analytical column, 4.6 mm x 100 mm, 3.5 &#181;m; flow rate = 1 ml/min), and visualized at 220 nm, the absorbance of amide backbone. Figure 5a and 5b shows that the compounds are homogeneous.
<img alt="Figure 5" src="/files/ftp_upload/54750/54750fig5.jpg" /> 
Figure 5. Analytical HPLC for the purified peptoids (a) and (b). There is a gradient 0-100% B over 30 minutes, column oven at 40 &#176;C (A = 95% H2O, 5% MeCN, 0.05% TFA; B = 95% MeCN, 5% H2O, 0.03% TFA). <a href="https://www.jove.com/files/ftp_upload/54750/54750fig5large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
The purified peptoids (a) and (b) were tested in cytotoxicity assays against L. mexicana axenic amastigotes. Frozen stocks of L. mexicana were defrosted and transformed to the amastigote stage ready for the assay. 72 hours after defrosting, the parasites should be insect stage promastigotes in their procyclic form, in log phase with many dividing cells. At this stage the parasites can be transformed into the amastigote stage using the pH and temperature shift described.27 At Day 9 of the transformation, the parasites will be in the non-replicating infectious metacyclic promastigote stage. Finally at Day 14, the parasites should be in the pathogenic amastigote stage where parasites lack the characteristic flagella of the promastigotes.28
5 mM stock solutions of the compounds were made in cell culture grade DMSO and tested in triplicates on a minimum of two occasions to ensure a robust data set was collected. A representative 96-well plate plan is shown in Figure 6. At the end of the assay, the cell viability reagent was added to each well and the fluorescence was measured as described to calculate the viability of parasites at each concentration tested (see Figure 7). ED50 values were calculated as peptoid (a) &#62;100 &#181;M and peptoid (b) 37 &#181;M respectively. The error bars plotted, show the variation between wells as a standard deviation and it can be seen that these are reasonable for most bars.
<img alt="Figure 6" src="/files/ftp_upload/54750/54750fig6.jpg" /> 
Figure 6. Representative 96 well plate plan for a cytotoxicity assay on 2 peptoid solutions (including positive and negative controls). med+ = medium (Schneider&#39;s Insect Medium, pH 5.5, 20% FBS, 1% P/S). L. mex. = 8 x 106/ml parasite culture in med+. AmphoB = 5 mM in DMSO. Peptoid = 5 mM stock solution in DMSO. Empty wells should contain sterile water. <a href="https://www.jove.com/files/ftp_upload/54750/54750fig6large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 7" src="/files/ftp_upload/54750/54750fig7.jpg" /> 
Figure 7. Results from cytotoxicity assay against L. mexicana axenic amastigote parasites using peptoid (a) and (b).&#160;It can be seen that the peptoid (b) is more effective than peptoid (a) at reducing the percentage of viable parasites, with both compounds having a dose dependent effect. The error bars plotted show the variation between wells as a standard deviation. <a href="https://www.jove.com/files/ftp_upload/54750/54750fig7large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Watch this Scientific Journal Video about An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity at JoVE.com

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

A protocol to synthesize peptoids with mixed cationic functionality in the same sequence is presented (lysine- and arginine-type monomers). Subsequent testing of these compounds against Leishmania mexicana, the protozoan parasites that cause cutaneous leishmaniasis, is also described.

This protocol describes the manual solid-phase synthesis of linear peptoids that contain two differently functionalized cationic monomers. In this ...

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Research

JoVE Journal

Biochemistry

12.0K Views.  Durham University. A protocol to synthesize peptoids with mixed cationic functionality in the same sequence is presented (lysine- and arginine-type monomers). Subsequent testing of these compounds against Leishmania mexicana, the protozoan parasites that cause cutaneous leishmaniasis, is also described.

Video: An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Peptidomimetics are great sources of protein ligands. The oligomeric nature of these compounds enables us to access large synthetic libraries on solid phase by using combinatorial chemistry. One of the most well studied classes of peptidomimetics is peptoids. Peptoids are easy to synthesize and have been shown to be proteolysis-resistant and cell-permeable. Over the past decade, many useful protein ligands have been identified through screening of peptoid libraries. However, most of the ligands identified from peptoid libraries do not display high affinity, with rare exceptions. This may be due, in part, to the lack of chiral centers and conformational constraints in peptoid molecules. Recently, we described a new synthetic route to access peptide tertiary amides (PTAs). PTAs are a superfamily of peptidomimetics that include but are not limited to peptides, peptoids and N-methylated peptides. With side chains on both &#945;-carbon and main chain nitrogen atoms, the conformation of these molecules are greatly constrained by sterical hindrance and allylic 1,3 strain. (Figure 1) Our study suggests that these PTA molecules are highly structured in solution and can be used to identify protein ligands. We believe that these molecules can be a future source of high-affinity protein ligands. Here we describe the synthetic method combining the power of both split-and-pool and sub-monomer strategies to synthesize a sample one-bead one-compound (OBOC) library of PTAs.

Peptide tertiary amides (PTAs) are a superfamily of peptidomimetics that include but are not limited to peptides, peptoids and N-methylated peptides. Here we describe a synthetic method which combines&#160;both split-and-pool and sub-monomer strategies to synthesize a one-bead one-compound library of PTAs.

Peptidomimetics are great sources of protein ligands. The oligomeric nature of these compounds enables us to access large synthetic libraries on solid ...

Chemistry

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Peptoids are a novel class of biomimetic, non-natural, sequence-specific heteropolymers that resist proteolysis, exhibit potent biological activity, and fold into higher order nanostructures. Structurally similar to peptides, peptoids are poly N-substituted glycines, where the side chains are attached to the nitrogen rather than the alpha-carbon. Their ease of synthesis and structural diversity allows testing of basic design principles to drive de novo design and engineering of new biologically-active and nanostructured materials.
Here, a simple manual peptoid synthesis protocol is presented that allows the synthesis of long chain polypeptoids ( up to 50mers) in excellent yields. Only basic equipment, simple techniques (e.g. liquid transfer, filtration), and commercially available reagents are required, making peptoids an accessible addition to many researchers' toolkits. The peptoid backbone is grown one monomer at a time via the submonomer method which consists of a two-step monomer addition cycle: acylation and displacement. First, bromoacetic acid activated in situ with N,N'-diisopropylcarbodiimide acylates a resin-bound secondary amine. Second, nucleophilic displacement of the bromide by a primary amine follows to introduce the side chain. The two-step cycle is iterated until the desired chain length is reached. The coupling efficiency of this two-step cycle routinely exceeds 98% and enables the synthesis of peptoids as long as 50 residues. Highly tunable, precise and chemically diverse sequences are achievable with the submonomer method as hundreds of readily available primary amines can be directly incorporated.
 
Peptoids are emerging as a versatile biomimetic material for nanobioscience research because of their synthetic flexibility, robustness, and ordering at the atomic level. The folding of a single-chain, amphiphilic, information-rich polypeptoid into a highly-ordered nanosheet was recently demonstrated. This peptoid is a 36-mer that consists of only three different commercially available monomers: hydrophobic, cationic and anionic. The hydrophobic phenylethyl side chains are buried in the nanosheet core whereas the ionic amine and carboxyl side chains align on the hydrophilic faces. The peptoid nanosheets serve as a potential platform for membrane mimetics, protein mimetics, device fabrication, and sensors. Methods for peptoid synthesis, sheet formation, and microscopy imaging are described and provide a simple method to enable future peptoid nanosheet designs.

A simple and general manual peptoid synthesis method involving basic equipment and commercially available reagents is outlined, enabling peptoids to be easily synthesized in most laboratories. The synthesis, purification and characterization of an amphiphilic peptoid 36mer is described, as well as its self-assembly into highly-ordered nanosheets.

Peptoids are a novel class of biomimetic, non-natural, sequence-specific heteropolymers that resist proteolysis, exhibit potent biological activity, and ...

Bioengineering

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Protein-protein interactions (PPIs) are intimately involved in almost all biological processes and are linked to many human diseases. Therefore, there is a major effort to target PPIs in basic research and in the pharmaceutical industry. Protein-protein interfaces are usually large, flat, and often lack pockets, complicating the discovery of small molecules that target such sites. Alternative targeting approaches using antibodies have limitations due to poor oral bioavailability, low cell-permeability, and production inefficiency.
Using peptides to target PPI interfaces has several advantages. Peptides have higher conformational flexibility, increased selectivity, and are generally inexpensive. However, peptides have their own limitations including poor stability and inefficiency crossing cell membranes. To overcome such limitations, peptide cyclization can be performed. Cyclization has been demonstrated to improve peptide selectivity, metabolic stability, and bioavailability. However, predicting the bioactive conformation of a cyclic peptide is not trivial. To overcome this challenge, one attractive approach it to screen a focused library to screen in which all backbone cyclic peptides have the same primary sequence, but differ in parameters that influence their conformation, such as ring size and position.
We describe a detailed protocol for synthesizing a library of backbone cyclic peptides targeting specific parasite PPIs. Using a rational design approach, we developed peptides derived from the scaffold protein Leishmania receptor for activated C-kinase (LACK). We hypothesized that sequences in LACK that are conserved in parasites, but not in the mammalian host homolog, may represent interaction sites for proteins that are critical for the parasites&#39; viability. The cyclic peptides were synthesized using microwave irradiation to reduce reaction times and increase efficiency. Developing a library of backbone cyclic peptides with different ring sizes facilitates a systematic screen for the most biological active conformation. This method provides a general, fast, and facile way to synthesize cyclic peptides.

A simple and general method for the synthesis of cyclic peptides using microwave irradiation is outlined. This procedure enables the synthesis of backbone cyclic peptides with a collection of different conformations while retaining the side chains and the pharmacophoric moieties., and therefore, allows to screen for the bioactive conformation.

Protein-protein interactions (PPIs) are intimately involved in almost all biological processes and are linked to many human diseases. Therefore, there is ...

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

Mimicking and exploiting virus properties and physicochemical and physical characteristics holds promise to provide solutions to some of the world's most pressing challenges. The sheer range and types of viruses coupled with their intriguing properties potentially give endless opportunities for applications in virus-based technologies. Viruses have the ability to self- assemble into particles with discrete shape and size, specificity of symmetry, polyvalence, and stable properties under a wide range of temperature and pH conditions. Not surprisingly, with such a remarkable range of properties, viruses are proposed for use in biomaterials 9, vaccines 14, 15, electronic materials, chemical tools, and molecular electronic containers4, 5, 10, 11, 16, 18, 12.
In order to utilize viruses in nanotechnology, they must be modified from their natural forms to impart new functions. This challenging process can be performed through several mechanisms including genetic modification of the viral genome and chemically attaching foreign or desired molecules to the virus particle reactive groups 8. The ability to modify a virus primarily depends upon the physiochemical and physical properties of the virus. In addition, the genetic or physiochemical modifications need to be performed without adversely affecting the virus native structure and virus function. Maize rayado fino virus (MRFV) coat proteins self-assemble in Escherichia coli producing stable and empty VLPs that are stabilized by protein-protein interactions and that can be used in virus-based technologies applications 8. VLPs produced in tobacco plants were examined as a scaffold on which a variety of peptides can be covalently displayed 13. Here, we describe the steps to 1) determine which of the solvent-accessible cysteines in a virus capsid are available for modification, and 2) bioconjugate peptides to the modified capsids. By using native or mutationally-inserted amino acid residues and standard coupling technologies, a wide variety of materials have been displayed on the surface of plant viruses such as, Brome mosaic virus 3, Carnation mottle virus 12, Cowpea chlorotic mottle virus 6, Tobacco mosaic virus 17, Turnip yellow mosaic virus 1, and MRFV 13.

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

A method to analyze the solvent accessibility of the thiol group of cysteine residues of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) followed by a peptide cross-linking reaction is described. The method takes advantage of the availability of several chemical groups on the surface of the VLPs that can be targets for specific reactions.

Mimicking  and exploiting virus properties and physicochemical and physical  characteristics holds promise to provide solutions to some of  the world's ...

Immunology and Infection

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Peptoids are sequence-controlled peptide-mimicking oligomers consisting of N-alkylated glycine units. Among many potential applications, peptoids have been thought of as a type of molecular information storage. Mass spectrometry analysis has been considered the method of choice for sequencing peptoids. Peptoids can be synthesized via solid phase chemistry using a repeating two-step reaction cycle. Here we present a method to manually synthesize oligo-peptoids and to analyze the sequence of the peptoids using tandem mass spectrometry (MS/MS) techniques. The sample peptoid is a nonamer consisting of alternating N-(2-methyloxyethyl)glycine (Nme) and N-(2-phenylethyl)glycine (Npe), as well as an N-(2-aminoethyl)glycine (Nae) at the N-terminus. The sequence formula of the peptoid is Ac-Nae-(Npe-Nme)4-NH2, where Ac is the acetyl group. The synthesis takes place in a commercially available solid-phase reaction vessel. The rink amide resin is used as the solid support to yield the peptoid with an amide group at the C-terminus. The resulting peptoid product is subjected to sequence analysis using a triple-quadrupole mass spectrometer coupled to an electrospray ionization source. The MS/MS measurement produces a spectrum of fragment ions resulting from the dissociation of charged peptoid. The fragment ions are sorted out based on the values of their mass-to-charge ratio (m/z). The m/z values of the fragment ions are compared against the nominal masses of theoretically predicted fragment ions, according to the scheme of peptoid fragmentation. The analysis generates a fragmentation pattern of the charged peptoid. The fragmentation pattern is correlated to the monomer sequence of the neutral peptoid. In this regard, MS analysis reads out the sequence information of the peptoids.

A protocol is described for the manual synthesis of oligo-peptoids followed by sequence analysis by mass spectrometry.

Peptoids are sequence-controlled peptide-mimicking oligomers consisting of N-alkylated glycine units. Among many potential applications, peptoids have ...

Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles (PPAs) and Related Biomaterials

Polyamine-based Peptide Amphiphiles (PPAs) are a new class of self-assembling amphiphilic biomaterials-related to the peptide amphiphiles (PAs). Traditional PAs possess charged amino acids as solubilizing groups (lysine, arginine), which are directly connected to a lipid segment or can contain a linker region made of neutral amino acids. Tuning the peptide sequence of PAs can yield diverse morphologies. Similarly, PPAs possess a hydrophobic segment and neutral amino acids, but also contain polyamine molecules as water solubilizing (hydrophilic) groups. As is the case with PAs, PPAs can also self-assemble into diverse morphologies, including small rods, twisted nano-ribbons, and fused nano-sheets, when dissolved in water. However, the presence of both primary and secondary amines on a single polyamine molecule poses a significant challenge when synthesizing PPAs. In this paper, we show a simple protocol, based on literature precedents, to achieve a facile synthesis of PPAs using solid phase peptide synthesis (SPPS). This protocol can be extended to the synthesis of PAs and other similar systems. We also illustrate the steps that are needed for cleavage from the resin, identification, and purification.

Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles PPAs and Related Biomaterials

The synthesis of polyamine-based peptide amphiphiles (PPAs) is a significant challenge due to the presence of multiple amine nitrogens, which requires judicious use of protecting groups to mask these reactive functionalities. In this paper, we describe a facile method for the preparation of these new class of self-assembling molecules.

Polyamine-based Peptide Amphiphiles (PPAs) are a new class of self-assembling amphiphilic biomaterials-related to the peptide amphiphiles (PAs). ...

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

This protocol presents the use of Lewis acidic multi-role reagents to circumvent kinetic trapping observed during the self-assembly of information-encoded oligomeric strands mediated by paired dynamic covalent interactions in a manner mimicking the thermal cycling commonly employed for the self-assembly of complementary nucleic acid sequences. Primary amine monomers bearing aldehyde and amine pendant moieties are functionalized with orthogonal protecting groups for use as dynamic covalent reactant pairs. Using a modified automated peptide synthesizer, the primary amine monomers are encoded into oligo(peptoid) strands through solid-phase submonomer synthesis. Upon purification by high-performance liquid chromatography (HPLC) and characterization by electrospray ionization mass spectrometry (ESI-MS), sequence-specific oligomers are subjected to high-loading of a Lewis acidic rare-earth metal triflate which both deprotects the aldehyde moieties and affects the reactant pair equilibrium such that strands completely dissociate. Subsequently, a fraction of the Lewis acid is extracted, enabling annealing of complementary sequence-specific strands to form information-encoded molecular ladders characterized by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The simple procedure outlined in this report circumvents kinetic traps commonly experienced in the field of dynamic covalent assembly and serves as a platform for the future design of robust, complex architectures.

A protocol is presented for the synthesis of information-encoded peptoid oligomers and for the sequence-directed self-assembly of these peptoids into molecular ladders using amines and aldehydes as dynamic covalent reactant pairs and Lewis acidic rare-earth metal triflates as multi-role reagents.

This protocol presents the use of Lewis acidic multi-role reagents to circumvent kinetic trapping observed during the self-assembly of information-encoded ...

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

In recent years, cyclic peptides have attracted increasing attention in the field of drug discovery due to their excellent biological activities, and, as a consequence, they are now used clinically. It is, therefore, critical to seek effective strategies for synthesizing cyclic peptides to promote their application in the field of drug discovery. This paper reports a detailed protocol for the efficient synthesis of cyclic peptides using on-resin or intramolecular (intermolecular) bisalkylation. Using this protocol, linear peptides were synthesized by taking advantage of solid-phase peptide synthesis with cysteine (Cys) and methionine (Met) coupled simultaneously on the resin. Further, cyclic peptides were synthesized via bisalkylation between Met and Cys using a tunable tether and an on-tether sulfonium center. The whole synthetic route can be divided into three major processes: the deprotection of Cys on the resin, the coupling of the linker, and the cyclization between Cys and Met in a trifluoroacetic acid (TFA) cleavage solution. Furthermore, inspired by the reactivity of the sulfonium center, a propargyl group was attached to the Met to trigger thiol-yne addition and form a cyclic peptide. After that, the crude peptides were dried and dissolved in acetonitrile, separated, and then purified by high-performance liquid chromatography (HPLC). The molecular weight of the cyclic peptide was confirmed by liquid chromatography-mass spectrometry (LC-MS), and the stability of the cyclic peptide combination with the reductant was further confirmed using HPLC. In addition, the chemical shift in the cyclic peptide was analyzed by 1H nuclear magnetic resonance (1H NMR) spectra. Overall, this protocol aimed to establish an effective strategy for synthesizing cyclic peptides.

This protocol presents the synthesis of cyclic peptides via bisalkylation between cysteine and methionine and the facile thiol-yne reaction triggered by the propargyl sulfonium center.

In recent years, cyclic peptides have attracted increasing attention in the field of drug discovery due to their excellent biological activities, and, as ...

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

In 1962, R.B. Merrifield published the first procedure using solid-phase peptide synthesis as a novel route to efficiently synthesize peptides. This technique quickly proved advantageous over its solution-phase predecessor in both time and labor. Improvements concerning the nature of solid support, the protecting groups employed and the coupling methods employed over the last five decades have only increased the usefulness of Merrifield's original system. Today, use of a Boc-based protection and base/nucleophile cleavable resin strategy or Fmoc-based protection and acidic cleavable resin strategy, pioneered by R.C. Sheppard, are most commonly used for the synthesis of peptides1.
Inspired by Merrifield's solid supported strategy, we have developed a Boc/tert-butyl solid-phase synthesis strategy for the assembly of functionalized bis-peptides2, which is described herein. The use of solid-phase synthesis compared to solution-phase methodology is not only advantageous in both time and labor as described by Merrifield1, but also allows greater ease in the synthesis of bis-peptide libraries. The synthesis that we demonstrate here incorporates a final cleavage stage that uses a two-step "safety catch" mechanism to release the functionalized bis-peptide from the resin by diketopiperazine formation.
Bis-peptides are rigid, spiro-ladder oligomers of bis-amino acids that are able to position functionality in a predictable and designable way, controlled by the type and stereochemistry of the monomeric units and the connectivity between each monomer. Each bis-amino acid is a stereochemically pure, cyclic scaffold that contains two amino acids (a carboxylic acid with an &alpha;-amine)3,4. Our laboratory is currently investigating the potential of functional bis-peptides across a wide variety of fields including catalysis, protein-protein interactions and nanomaterials.

The efficient solid-phase peptide synthesis of a functionalized bis-peptide trimer utilizing a "safety catch" cleavage procedure from HMBA resin is described.

In 1962, R.B. Merrifield published the first procedure using solid-phase peptide synthesis as a novel route to efficiently synthesize peptides. This ...

Biology

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Lysine methylation is an emerging post-translation modification and it has been identified on several histone and non-histone proteins, where it plays crucial roles in cell development and many diseases. Approximately 5,000 lysine methylation sites were identified on different proteins, which are set by few dozens of protein lysine methyltransferases. This suggests that each PKMT methylates multiple proteins, however till now only one or two substrates have been identified for several of these enzymes. To approach this problem, we have introduced peptide array based substrate specificity analyses of PKMTs. Peptide arrays are powerful tools to characterize the specificity of PKMTs because methylation of several substrates with different sequences can be tested on one array. We synthesized peptide arrays on cellulose membrane using an Intavis SPOT synthesizer and analyzed the specificity of various PKMTs. Based on the results, for several of these enzymes, novel substrates could be identified. For example, for NSD1 by employing peptide arrays, we showed that it methylates K44 of H4 instead of the reported H4K20 and in addition H1.5K168 is the highly preferred substrate over the previously known H3K36. Hence, peptide arrays are powerful tools to biochemically characterize the PKMTs.

Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

Lysine methylation is an emerging post-translation modification and it has been identified on several histone and non-histone proteins, where it plays ...

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

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Przeglądaj więcej filmów

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles (PPAs) and Related Biomaterials

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays