Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

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9.0K Views

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10:57 min

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February 7th, 2017

DOI :

10.3791/54994-v

February 7th, 2017

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Yolanda Blanco¹, Mercedes Moreno-Paz¹, Victor Parro¹

¹Department of Molecular Evolution, Centro de Astrobiología (CAB, INTA-CSIC)

Podsumowanie

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Keywords Cyanobacteria

Antibody Microarray

Fluorescence Microarray Immunoassay

Environmental Monitoring

Water Management

Toxin Detection

Biodiversity

Agricultural Monitoring

Planetary Exploration

Sample Processing

Antibody Labeling

CYANOCHIP

Protein Printing Buffer

Rozdziały w tym wideo

0:05

Title

1:11

Fluorescence Antibody Labeling

2:05

CYANOCHIP Production

3:13

Preparation of Environmental Multi-analyte Extracts for Fluorescent Sandwich Microarray Immunoassay

4:42

Fluorescent Sandwich Microarray Immunoassay

5:20

Incubation of Multi-analyte Sample Extract with Microarray and Washing

6:42

Fluorescent Antibodies and Scanning Fluorescence

7:53

Results: Cyanobacteria Detection Using the CYANOCHIP Multiplex Immunoassay

9:17

Conclusion

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