Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

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18.8K Views

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08:43 min

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January 24th, 2017

DOI :

10.3791/55166-v

January 24th, 2017

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Matthew McKenzie¹^,², Sze C. Lim¹, Michael R. Duchen³

¹Centre for Genetic Diseases, Hudson Institute of Medical Research, ²The Department of Molecular and Translational Sciences, Monash University, ³Department of Physiology, University College London

Podsumowanie

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Keywords Mitochondrial Calcium

Mitochondrial Membrane Potential

Fluorescent Microscopy

Live Cell Imaging

Calcium Regulation

Mitochondrial Disease

Rozdziały w tym wideo

0:05

Title

0:54

Preparation of Cells

2:21

Staining of Cells with TMRM and Fluo-4 AM

3:46

Cell Imaging and Analysis

6:44

Results: Measuremnt of Mitochondrial Membrane Potential and Calcium in Digitonin Permeabilized 143B Cells

7:57

Conclusion

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