Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture

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11.6K Views

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08:53 min

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May 10th, 2017

DOI :

10.3791/55442-v

May 10th, 2017

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Lin Jiajia*¹, Mak Shinghung*², Zheng Jiacheng¹, Wang Jialing¹, Xu Dilin¹, Hu Shengquan², Zhang Zaijun³, Wang Qinwen¹, Han Yifan²^,⁴, Cui Wei¹^,²

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, ²Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, ³Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, ⁴International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong

Podsumowanie

Rozdziały w tym wideo

0:05

Title

8:19

Conclusion

7:08

Results: Double and Triple Stainings in CGN Culture

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Tissue Harvest from 8 Day Old Sprague-Dawley Rats

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FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture

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Assessment of Neuronal Viability

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