Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
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08:53 min
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May 10th, 2017
DOI :
May 10th, 2017
•Lin Jiajia*1, Mak Shinghung*2, Zheng Jiacheng1, Wang Jialing1, Xu Dilin1, Hu Shengquan2, Zhang Zaijun3, Wang Qinwen1, Han Yifan2,4, Cui Wei1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
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Chapters in this video
0:05
Title
0:46
Tissue Harvest from 8 Day Old Sprague-Dawley Rats
3:51
FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
5:30
Assessment of Neuronal Viability
7:08
Results: Double and Triple Stainings in CGN Culture
8:19
Conclusion
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