JoVE Logo

Zaloguj się

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

9.4K Views

10:58 min

July 27th, 2017

DOI :

10.3791/55869-v

July 27th, 2017


Przeglądaj więcej filmów

Protein phosphoinositide Interactions

Rozdziały w tym wideo

0:05

Title

1:04

Fabricating Micropatterned PDMS Blocks

9:56

Conclusion

8:49

Results: Assessing PLC-δ1 PH Domain: Phosphoinositide Interaction with PIP-on-a-chip Assay

2:14

Preparing Small Unilamellar Vesicles (SUVs)

3:29

Assembling the Microfluidic Device

4:42

Forming Supported Lipid Bilayers (SLBs)

7:56

Testing PLC-δ1 PH Domain Interction with PI(4,5)P2-containing SLBs

Powiązane Filmy

article

10:13

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

15.1K Views

article

07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

9.4K Views

article

11:08

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

15.9K Views

article

13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

16.9K Views

article

10:41

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

8.2K Views

article

10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

10.3K Views

article

08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

8.5K Views

article

08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

3.9K Views

article

02:23

Test modulacji pH na podpartych dwuwarstwach lipidowych w celu wykrycia interakcji białko-fosfoinozytyd

89 Views

article

01:42

Fosfoinozytydy i

7.3K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone