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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

12.4K Views

11:35 min

June 16th, 2017

DOI :

10.3791/55903-v

June 16th, 2017


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CRISPR Cas9

Rozdziały w tym wideo

0:05

Title

10:40

Conclusion

8:59

Results: Knockout Cell Lines Generated by Two CRISPER/Cas9 Methods

2:31

Isolation of Clonal Populations

0:57

Transfection of CRISPER Component into Cultured Cells and Drug Selection

3:53

Screening Candidates by Dot Blot

6:30

Screening Candidates by Colony PCR

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