Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Please note that all translations are automatically generated. Click here for the English version.
21.8K Views
•
11:27 min
•
August 28th, 2018
DOI :
August 28th, 2018
•Rozdziały w tym wideo
0:05
Title
1:09
Preparation of CRISPR-reagents for Embryo Microinjection
4:03
Microinjection of CRISPR-components into Zebrafish Embryos
6:12
Analysis of Efficiency of Indel Formation Using an HMA
8:45
Identification and Propagation of Knock-out Lines
9:45
Results: Zebrafish Embryos Exhibit a Pigment Defect when Injected with an sgRNA Targeting Tyrosinase at the One Cell Stage
10:32
Conclusion
Powiązane Filmy
09:57
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
10.5K Views
11:35
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
12.4K Views
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
15.0K Views
06:11
Production and Purification of Baculovirus for Gene Therapy Application
14.6K Views
08:27
Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
13.3K Views
09:55
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
25.9K Views
10:21
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
9.5K Views
09:06
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
10.1K Views
07:20
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
7.5K Views
08:54
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
7.0K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone