Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

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12.8K Views

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08:21 min

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August 15th, 2017

DOI :

10.3791/56142-v

August 15th, 2017

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Thomas R. Vincent¹, James Canham¹, Masatsugu Toyota²^,³^,⁴, Marieta Avramova¹, Sam T. Mugford⁵, Simon Gilroy², Anthony J. Miller¹, Saskia Hogenhout⁵, Dale Sanders¹

¹Department of Metabolic Biology, John Innes Centre, Norwich Research Park, ²Department of Botany, University of Wisconsin, Madison, ³Department of Biochemistry and Molecular Biology, Saitama University, ⁴Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), ⁵Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

Podsumowanie

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Keywords Arabidopsis

Calcium Signals

Insect Feeding

Fluorescent Biosensor

In Vivo Recording

Plant aphid Interactions

Calcium Signaling

GCaMP3

96 well Plate

Fluorescent Stereo Microscope

Image Analysis

Fiji

ImageJ

Rozdziały w tym wideo

0:05

Title

0:44

Arabidopsis Leaf Detachment

1:38

Fluorescence Microscopy

3:06

Data Collection

5:39

Time-course Video Creation

6:39

Results: Analysis of GCaMP3 Fluorescence to Detect Aphid-elicited Calcium Signals at the Feeding Site in Detached Arabidopsis Leaves

7:30

Conclusion

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