Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
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06:29 min
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July 30th, 2020
DOI :
July 30th, 2020
•Rozdziały w tym wideo
0:04
Introduction
1:13
Cell Synchronization
1:42
FRET/Fluorescence Lifetime Imaging Microscopy (FLIM) Acquisitions
2:50
Donor/Acceptor Pair Delta Lifetime Calculation and FLIM Value Comparison
3:53
Results: Representative Aurora Kinase A (AURKA) FRET Biosensor and Negative Control Images
5:52
Conclusion
0:36
Donor/Acceptor Förster Resonance Energy Transfer (FRET) Pair Selection
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