Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
Please note that all translations are automatically generated. Click here for the English version.
8.7K Views
•
14:05 min
•
October 27th, 2017
DOI :
October 27th, 2017
•Kayla Huemer*1,2, Jayne M. Squirrell*3, Robert Swader2, Kirsten Pelkey2, Danny C. LeBert4, Anna Huttenlocher5,6, Kevin W. Eliceiri1,2,3
1Department of Biomedical Engineering, University of Wisconsin-Madison, 2Morgridge Institute for Research, University of Wisconsin-Madison, 3Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 4Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, 5Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 6Department of Pediatrics, University of Wisconsin-Madison
Rozdziały w tym wideo
0:05
Title
0:57
Design and 3D Printing of Molds
2:57
PDMS Fabrication of zWEDGI Device
4:43
Plasma-bonding zWEDGI to Glass Dish
7:19
Channel Preparation and Loading Larvae
9:52
Wounding and Imaging Larvae and End of Experiment
11:14
Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin
12:36
Conclusion
Powiązane Filmy
08:52
Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin
11.9K Views
06:55
A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
11.3K Views
12:21
Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
14.1K Views
07:40
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
9.5K Views
09:28
Automated High-throughput Behavioral Analyses in Zebrafish Larvae
15.2K Views
08:55
A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
8.8K Views
11:41
Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
1.9K Views
09:39
Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
14.6K Views
07:07
4D Light-sheet Imaging of Zebrafish Cardiac Contraction
1.1K Views
05:25
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
4.6K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone