A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

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14.5K Views

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13:35 min

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March 1st, 2018

DOI :

10.3791/56445-v

March 1st, 2018

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Li Xie¹, Liqin Chen³, Pan Gu⁴, Lanlan Wei¹, Xuejun Kang²

¹School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, ²Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, ³School of Public Health, Tianjin Medical University, ⁴British Columbia Academy, Nanjing Foreign Language School

Podsumowanie

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Keywords High pressure Liquid Chromatography

Catecholamine Neurotransmitters

Metabolites

Solid phase Extraction

Electrochemical Detection

Monoamine Neurotransmitters

Rozdziały w tym wideo

0:04

Title

1:27

Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines

4:44

Preparation of Real Urine Samples and Mobile Phase

6:25

PFSPE Extraction and HPLC Analysis

9:58

Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine

11:43

Conclusion

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