Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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13:14 min
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February 6th, 2018
DOI :
February 6th, 2018
•Rozdziały w tym wideo
0:04
Title
1:17
Isolation of Human Peripheral Blood Mononuclear Cells
2:00
Tetramer-Associated Magnetic Enrichment of Antigen Specific B-Cells
3:58
Staining of Antigen Specific Human B-Cells and Cell Sorting
4:56
Single Cell RT-PCR
6:00
Screening and Verification of Heavy and Light Chain Expressing Vectors
7:45
Production of Monoclonal Antibodies
10:33
Results: Detection of Relevant Antigen Specific B-Cells and Generation of Highly Discriminative Monoclonal Antibody from Donor Peripheral Blood Mononuclear Cells
12:41
Conclusion
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