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Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

14.0K Views

09:35 min

February 2nd, 2018

DOI :

10.3791/56613-v

February 2nd, 2018


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In Vivo

Rozdziały w tym wideo

0:05

Title

0:52

Recombinatorial Cloning to Generate Ready-for-injection Clones

2:23

Hydrodynamic Tail Vein Injection

4:22

Induction of Transfected CreER with Tamoxifen

5:33

Induction of Tetracycline-dependent Gene or shRNA Expression

6:35

Preparation of Mouse Liver for Analysis by Immunostaining

7:30

Results: Constitutive and Inducible Gene Expression in the Liver Following HTVI

8:47

Conclusion

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