Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

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7.5K Views

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11:20 min

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March 29th, 2018

DOI :

10.3791/56616-v

March 29th, 2018

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Claudia Siverino¹, Barbara Tabisz², Tessa Lühmann³, Lorenz Meinel³, Thomas Müller⁴, Heike Walles¹^,², Joachim Nickel¹^,²

¹Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg 'Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung', Institutsteil Würzburg, ²Lehrstuhl für Tissue Engineering und Regenerative Medizin, Universitätsklinikum Würzburg, ³Lehrstuhl für Pharmazeutische Technologie und Biopharmazie, Universität Würzburg, ⁴Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg

Podsumowanie

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Keywords Site directed

Immobilization

Bone Morphogenetic Protein 2

BMP2

Click Chemistry

Osteogenic Scaffold

Non healing Long Bone Defects

Covalent Coupling

Absorption

Encapsulation

Recombinant Protein Expression

Rozdziały w tym wideo

0:04

Title

0:46

Expression and Purification of BMP2-K3Plk

5:46

Covalent Coupling Technique of BMP2-K3Plk to Azide Functionalized Agarose Beads

7:04

Measuring Alkaline Phosphatase (ALP) Expression to Prove the In Vitro Bioactivity of the Produced BMP2-K3Plk Before and After the Coupling Reaction

8:34

Results: Evaluation of the Bioactivity of BMP2-K3Plk after Site-Directed Immobilization

9:54

Conclusion

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