JoVE Logo

Zaloguj się

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

10.5K Views

13:03 min

March 8th, 2018

DOI :

10.3791/56837-v

March 8th, 2018


Przeglądaj więcej filmów

DNA Isolation

Rozdziały w tym wideo

0:04

Title

0:58

DNA Extraction

4:39

DNA Shearing

5:28

Bead Clean-up

7:06

Library Preparation

7:40

Adaptor Ligation

8:46

PCR Amplification

9:12

Size Selection for Desired Library Size and Quality Control

11:04

Results: Isolation and High-throughput Sequencing of Poor Quality DNA Isolated from Various Herbarium Species

12:33

Conclusion

Powiązane Filmy

article

13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

10.5K Views

article

06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

11.6K Views

article

12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

12.8K Views

article

08:36

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

9.6K Views

article

10:28

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

8.6K Views

article

12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

4.9K Views

article

08:55

Application of DNA Barcoding to Identify Medicinal Plants

1.2K Views

article

09:19

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices

8.9K Views

article

10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

22.6K Views

article

08:53

Isolation and Transcriptome Analysis of Plant Cell Types

1.3K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone