An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

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24.2K Views

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09:35 min

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February 26th, 2018

DOI :

10.3791/57038-v

February 26th, 2018

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Kavi Devraj¹^,², Sylvaine Guérit¹, Jakranka Macas¹, Yvonne Reiss¹

¹Institute of Neurology (Edinger Institute), Goethe University Hospital, ²Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital

Podsumowanie

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Neurological Disorders

Brain Edema

Blood brain Barrier Dysfunction

Cardiac Perfusion

Fluorometry

Fluorescence Microscopy

Rozdziały w tym wideo

0:04

Title

1:15

Administration of Tracers

2:01

Blood Collection and Cardiac Perfusion

3:36

Organ Collection and Storage

5:01

Homogenization and Centrifugation

6:38

Immunofluorescence Visualization of Tracer

7:59

Results: Tracer Clearance at Short and Long Circulation Times

8:51

Conclusion

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