Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

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9.2K Views

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12:29 min

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April 16th, 2018

DOI :

10.3791/57547-v

April 16th, 2018

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Xiaomin Dong¹, Raquel Cuevas-Diaz Duran¹, Yanan You¹, Jia Qian Wu¹

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

Podsumowanie

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Keywords Transcription Factor Olig2

PDGFR Cells

ChIP seq

Low cell ChIP seq

Oligodendrocyte Precursor Cells

Immunopanning

Genome wide Transcriptional Regulation

Epigenetic Mechanisms

Cell Differentiation

Cell Development

Rozdziały w tym wideo

0:04

Title

1:10

Purification of PDGFRα Positive Oligodendrocyte Lineage Cells from Mouse

5:03

Low-cell ChIP Preparation and ChIP Library Construction for High-throughput Sequencing

9:41

Results: Use of ChIP Analysis to Study Genome-wide Binding of the Olig2 in OPCs

11:42

Conclusion

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