Purification of PDGFRα Positive Oligodendrocyte Lineage Cells from Mouse
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Low-cell ChIP Preparation and ChIP Library Construction for High-throughput Sequencing
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Results: Use of ChIP Analysis to Study Genome-wide Binding of the Olig2 in OPCs
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Conclusion
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The overall goal of this experiment is to analyze the genome-wide binding of oligodendrocyte transcription factor two in acutely purified brain oligodendrocyte precursor cells by performing immunopanning, low-cell chromatin immunoprecipitation, hi
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Here we present a protocol which is designed to analyze the genome-wide binding of the oligodendrocyte transcription factor 2 (Olig2) in acutely purified brain oligodendrocyte precursor cells (OPCs) by performing low-cell chromatin immunoprecipitation (ChIP), library preparation, high-throughput sequencing and bioinformatic data analysis.