<h2 class="jove_title"><a href="https://www.jove.com/video/51272">Folding and Characterization of a Bio-responsive Robot from DNA Origami</a></h2>Yaniv Amir, Almogit Abu-Horowitz, Ido BacheletFaculty of Life Sciences and the Institute for Nanotechnology &amp; Advanced Materials, Bar-Ilan UniversityDNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here we describe a protocol for the folding of a bio-responsive robot from DNA origami, its purification and negative staining for transmission electron microscopic imaging (TEM). <h2 class="jove_title"><a href="https://www.jove.com/video/53109">An Unpredictable Chronic Mild Stress Protocol for Instigating Depressive Symptoms, Behavioral Changes and Negative Health Outcomes in Rodents</a></h2>Jefferson C. Frisbee1, Steven D. Brooks1, Shyla C. Stanley1, Alexandre C. d'Audiffret21Department of Physiology and Pharmacology, West Virginia University Health Sciences Center, 2Division of Vascular and Endovascular Surgery, Center for Cardiovascular and Respiratory Sciences, West Virginia University Health Sciences CenterThe unpredictable chronic mild stress (UCMS) protocol is a validated method for studying behavioral and physiological changes associated with chronic stress and depressive symptoms. Eight weeks of imposition of the UCMS protocol induces behavioral changes and poor health outcomes in rodents of either gender.<h2 class="jove_title"><a href="https://www.jove.com/video/53313">Nasal Wipes for Influenza A Virus Detection and Isolation from Swine</a></h2>Jacqueline M. Nolting, Christine M. Szablewski, Jody L. Edwards, Sarah W. Nelson, Andrew S. BowmanDepartment of Veterinary Preventive Medicine, The Ohio State UniversityThe authors present a protocol to collect swine nasal wipes to detect and isolate influenza A viruses. <h2 class="jove_title"><a href="https://www.jove.com/video/53416">Measurement of H2S in Crude Oil and Crude Oil Headspace Using Multidimensional Gas Chromatography, Deans Switching and Sulfur-selective Detection</a></h2>Nicole E. Heshka, Darcy B. HagerCanmetENERGY, Natural Resources CanadaA multidimensional gas chromatography method for the analysis of dissolved hydrogen sulfide in liquid crude oil samples is presented. A Deans switch is used to heart-cut light sulfur gases for separation on a secondary column and detection on a sulfur chemiluminescence detector.

december 2015 - this month in jove: flu surveillance in swine, dna nanorobots, analyzing crude oil, and a translational model of depression

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/106">December 2015 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/i2">JoVE Immunology &amp; Infection</a>, we know that the peak of flu season is on its way-and because pigs are important hosts for the influenza A virus, it is critical to monitor virus evolution in swine populations to get a snapshot of current circulating strains. Nasal swabs are a gold-standard technique for taking diagnostic samples from live pigs, but it requires restraining the pigs. <a href="http://www.jove.com/video/53313">Nolting et al.</a> present an alternative sampling method using nasal wipes, which involves rubbing a piece of fabric across the snout of the pig with minimal to no restraint of the animal. The nasal wipe procedure is simple to perform, and virus detection and isolation rates are adequate to make it a viable and low-stress sampling method for flu in pigs.
In <a href="http://www.jove.com/chem">JoVE Chemistry</a>, DNA nanorobots are hollow hexagonal nanodevices that open in response to specific stimuli to present the cargo sequestered inside. This month, <a href="http://www.jove.com/video/51272">Amir et al.</a> describe a nanorobot fabrication protocol that uses the DNA origami technique. This involves mixing short single-stranded DNA staples with long, circular, single-stranded DNA scaffolds. In a standard thermocycler, the staples anneal to the scaffolds, thus driving the folding of the nanorobot. After the folding reaction is complete, agarose gel electrophoresis (AGE) is used to visualize and estimate the purity of the DNA nanorobots. Finally, nanorobot fabrication is verified by transmission electron microscopy (TEM). This procedure allows for the construction of complex 2D/3D nano-architecture.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, crude oil is one of the most valuable natural resources because it is the raw material for many fuels and chemical products. Accurate analysis of crude oil, especially the content of highly toxic hydrogen sulfide (H2S), is essential for developing and implementing safety measures in the event of a release or spill. <a href="http://www.jove.com/video/53416/measurement-h2s-crude-oil-crude-oil-headspace-using-multidimensional">Heshka and Hager</a> present a multidimensional gas chromatography method for analyzing dissolved hydrogen sulfide in crude oil samples. As far as we know, this is the only method that can accurately measure dissolved hydrogen sulfide in heavy crudes, without the use of sub-ambient cooling.
In <a href="http://www.jove.com/behavior">JoVE Behavior</a>, <a href="http://www.jove.com/video/53109">Frisbee et al.</a> present one of the most useful models for studying the pathophysiology of depression in rodents. This protocol uses a variety of mild stressors, and after a period of exposure to unpredictable chronic mild stress, rodents develop behaviors and cardiovascular alterations that are comparable to the symptoms of clinical depression. This approach allows for detailed studies of the pathological mechanisms of chronic stress, and can be used to test new therapies and interventions for chronic stress-induced depression.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/106">December 2015 issue</a> of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about December 2015 - This Month in JoVE: Flu Surveillance in Swine, DNA Nanorobots, Analyzing Crude Oil, and a Translational Model of Depression at JoVE.com

December 2015 - This Month in JoVE: Flu Surveillance in Swine, DNA Nanorobots, Analyzing Crude Oil, and a Translational Model of Depression

Here's a look at what's coming up in the December 2015 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Immunology &amp; Infection, we know ...

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2.0K Views.  Massachusetts Eye and Ear. December 2015 - This Month in JoVE: Flu Surveillance in Swine, DNA Nanorobots, Analyzing Crude Oil, and a Translational Model of Depression

Video: December 2015 - This Month in JoVE: Flu Surveillance in Swine, DNA Nanorobots, Analyzing Crude Oil, and a Translational Model of Depression

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...