Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

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13.6K Views

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11:00 min

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June 12th, 2018

DOI :

10.3791/57786-v

June 12th, 2018

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Sabyasachi Dash¹^,²^,³, Muthukumar Balasubramaniam², Chandravanu Dash², Jui Pandhare²

¹School of Biotechnology, Kalinga Institute of Industrial Technology University, ²1. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, ³Kalinga Institute of Industrial Technology University

Podsumowanie

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Keywords Biotinylated MiRNA Mimics

MRNA Targets

Pulldown Assay

Locked Nucleic Acids

Streptavidin coated Magnetic Beads

MiRNA mRNA Interactions

MiRNA Biology

MiRNA Therapeutics

Rozdziały w tym wideo

0:04

Title

1:34

Preparation of Streptavidin Coated Magnetic Beads — I

3:52

Preparation of Cell Lysate

5:36

Preparation of Streptavidin Magnetic Beads — II

6:34

Pull-down of Target mRNA-miRNA Complexes

7:35

Total RNA Extracted, cDNA Synthesis, and qPCR

8:57

Results: Use of MicroRNAs to Capture the Target mRNA

9:55

Conclusion

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