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Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

80.9K Views

09:18 min

September 7th, 2018

DOI :

10.3791/58154-v

September 7th, 2018


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Correlative Light Electron Microscopy CLEM

Rozdziały w tym wideo

0:04

Title

0:51

Patterned Sapphire Disc Preparation, Cell Culture, and Imaging

3:16

Cryo-immobilization and Freeze Substitution (FS)

5:24

Resin Embedding and Patterned Sapphire Disc Removal from the Polymerized Resin Block

6:43

Ultrathin Section Preparation and Imaging via Transmission Electron Microscopy (TEM)

8:06

Results: Tracking and Imaging of Viral Protein-associated Structures via Correlative Light Electron Microscopy (CLEM)

8:47

Conclusion

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