In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
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14.7K Views
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08:58 min
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May 1st, 2019
DOI :
May 1st, 2019
•Rozdziały w tym wideo
0:04
Title
0:42
DNA Template Preparation by PCR for In Vitro Transcription
3:20
mRNA Generation by In Vitro Transcription
4:22
Cells Transfection with mRNA
6:04
Luciferase Activity Measurement
7:29
Results: Translation Efficiency of a Fluc mRNA Determined by Luciferase Reporter Assay
8:21
Conclusion
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