<h2 class="jove_title"><a href="https://www.jove.com/video/56220">A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting</a></h2>Linda S. Franck1,2, Monica R. McLemore1,2, Norlissa Cooper1, Baylee De Castro3, Anastasia Y. Gordon4, Schyneida Williams5, Shanell Williams2, Larry Rand2,31<a href='/institutions/NA-North-America/US-United-States/CA-California/23335-University-of-California,-San-Francisco'>School of Nursing, University of California, San Francisco</a>, 2<a href='/institutions/NA-North-America/US-United-States/CA-California/23335-University-of-California,-San-Francisco'>UCSF California Preterm Birth Initiative, University of California, San Francisco</a>, 3<a href='/institutions/NA-North-America/US-United-States/CA-California/23335-University-of-California,-San-Francisco'>School of Medicine, University of California, San Francisco</a>, 4<a href='/institutions/NA-North-America/US-United-States/CA-California/26087-San-Francisco-Black-Infant-Health-Program'>San Francisco Black Infant Health Program</a>, 5<a href='/institutions/NA-North-America/US-United-States/CA-California/26088-Homeless-Prenatal-Program'>Homeless Prenatal Program, San Francisco, CA</a>This manuscript describes the Research Prioritization by Affected Communities (RPAC) protocol and findings from its use with women at risk for preterm birth. Using the protocol, women identified and prioritized their unanswered questions about pregnancy, birth and neonatal care aimed at influencing research priority setting by funders and researchers.<h2 class="jove_title"><a href="https://www.jove.com/video/56733">Frame-by-Frame Video Analysis of Idiosyncratic Reach-to-Grasp Movements in Humans</a></h2>Jenni M. Karl1, Jessica R. Kuntz2, Layne A. Lenhart2, Ian Q. Whishaw21<a href='/institutions/NA-North-America/CA-Canada/26427-Thompson-Rivers-University'>Department of Psychology, Thompson Rivers University</a>, 2<a href='/institutions/NA-North-America/CA-Canada/24784-University-of-Lethbridge'>Department of Neuroscience, University of Lethbridge</a>This protocol describes how to use frame-by-frame video analysis to quantify idiosyncratic reach-to-grasp movements in humans. A comparative analysis of reaching in sighted versus unsighted healthy adults is used to demonstrate the technique, but the method can also be applied to the study of developmental and clinical populations.<h2 class="jove_title"><a href="https://www.jove.com/video/56760">Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery</a></h2>Ming J. Cheng1, Priya Prabakaran1, Rajiv Kumar2,3, Srinivas Sridhar1,2,3, Eno E. Ebong1,4,51<a href='/institutions/NA-North-America/US-United-States/MA-Massachusetts/3887-Northeastern-University'>Department of Chemical Engineering, Northeastern University</a>, 2<a href='/institutions/NA-North-America/US-United-States/MA-Massachusetts/3887-Northeastern-University'>Nanomedicine Science and Technology Center, Northeastern University</a>, 3<a href='/institutions/NA-North-America/US-United-States/MA-Massachusetts/3887-Northeastern-University'>Department of Physics, Northeastern University</a>, 4<a href='/institutions/NA-North-America/US-United-States/MA-Massachusetts/3887-Northeastern-University'>Departments of Bioengineering, Northeastern University</a>, 5<a href='/institutions/NA-North-America/US-United-States/NY-New-York/26332-Albert-Einstein-College-of-Medicine'>Department of Neuroscience, Albert Einstein College of Medicine</a>We describe a method of synthesizing biocompatible 10-nm gold nanoparticles, functionalized by coating poly-ethylene glycol onto the surface. These particles can be used in vitro and in vivo for delivering therapeutics to nanoscale cellular and extracellular spaces that are difficult to access with conventional nanoparticle sizes.<h2 class="jove_title"><a href="https://www.jove.com/video/57324">Preparation, Purification, and Use of Fatty Acid-containing Liposomes</a></h2>Lin Jin*1,2, Aaron E. Engelhart*1,3, Katarzyna P. Adamala1,3, Jack W. Szostak11<a href='/institutions/NA-North-America/US-United-States/MA-Massachusetts/24866-Massachusetts-General-Hospital'>Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital</a>, 2<a href='/institutions/NA-North-America/US-United-States/MA-Massachusetts/22-Boston-University'>Department of Biomedical Engineering, Boston University</a>, 3<a href='/institutions/NA-North-America/US-United-States/MN-Minnesota/25170-University-of-Minnesota'>Department of Genetics, Cell Biology, and Development, University of Minnesota</a>Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.

jove monthly highlights: january 2018

Here&#39;s a look at what is featured in the January 2018 issue of <a href="https://www.jove.com/">JoVE</a>: The World&#39;s Premier Video Journal.
This month, JoVE <a href="https://www.jove.com/journal/behavior">Behavior</a> really grabs our attention with a <a href="https://www.jove.com/video/56733/frame-frame-video-analysis-idiosyncratic-reach-to-grasp-movements">novel protocol</a> to analyze reaching and grasping movements. Here, our authors used frame-by-frame video to study healthy adult subjects as they reach for a donut hole on a pedestal. This technique allows researchers to study these movements without specialized or expensive equipment.
From studying hands to extending one to communities in need, our <a href="https://www.jove.com/video/56220/a-novel-method-for-involving-women-color-at-high-risk-for-preterm">authors</a> in JoVE <a href="https://www.jove.com/journal/behavior">Behavior</a> describe a strategy to directly engage women at high socio-demographic risk for preterm birth. The Research Prioritization by Affected Communities protocol represents big step forward in the involvement of patients and the public in setting research priorities.
Next, as athletes across the globe prepare to compete for Olympic gold, JoVE <a href="https://www.jove.com/journal/bioengineering">Bioengineering</a> features a method to synthesize <a href="https://www.jove.com/video/56760/synthesis-functionalized-10-nm-polymer-coated-gold-particles-for">gold nanoparticles</a> for use in targeted drug delivery. After synthesis and purification, these fluorescent nanoparticles can be visualized both in vitro and in vivo.
Finally, JoVE <a href="https://www.jove.com/journal/bioengineering">Bioengineering</a> was honored to feature the <a href="https://www.jove.com/video/57324/preparation-purification-and-use-of-fatty-acid-containing-liposomes">work</a> of a Nobel laureate and colleagues, who share their protocol to prepare and purify liposomes. Using these liposomes, researchers can answer key questions related to synthetic biology and the origin of life.
You&#39;ve just had a snapshot of the January 2018 issue of JoVE. Visit our website to see the full-length articles, plus many more, in JoVE: The World&#39;s Premier Video Journal.

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JoVE Monthly Highlights: January 2018

Here&#39;s a look at what is featured in the January 2018 issue of JoVE: The World&#39;s Premier Video Journal.
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2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

JoVE Monthly Highlights: January 2018

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2012: A Year In Review