Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

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7.8K Views

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00:09 min

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February 18th, 2020

DOI :

10.3791/60726-v

February 18th, 2020

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Eileen F. Ablondi¹, Sudip Paudel², Morgan Sehdev³, John P. Marken⁴, Andrew D. Halleran⁴, Atiqur Rahman⁵, Peter Kemper⁵, Margaret S. Saha²

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, ²Department of Biology, College of William and Mary, ³Harvard Medical School, Harvard University, ⁴Department of Bioengineering, California Institute of Technology, ⁵Department of Computer Science, College of William and Mary

Podsumowanie

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Fluorescent Calcium Imaging

In Situ Hybridization

Neuronal Precursor Characterization

Xenopus Laevis

Calcium Activity Patterns

Embryonic Development

Sterile Techniques

Explant Transfer

Neural Tissue Isolation

Rozdziały w tym wideo

0:04

Introduction

0:34

Dissection and Imaging Plate Preparation

1:28

Embryo Dissection

5:27

Calcium Imaging

7:19

Results: Representative Fluorescent Calcium Imaging and Subsequent In Situ Hybridization

8:41

Conclusion

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