Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

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12.0K Views

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06:22 min

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August 25th, 2020

DOI :

10.3791/61542-v

August 25th, 2020

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Ashwin Narayanan¹, Enrique Blanco-Carmona², Engin Demirdizen¹, Xueyuan Sun¹^,³, Christel Herold-Mende⁴, Matthias Schlesner², Sevin Turcan¹

¹Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, ²Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), ³Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), ⁴Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

Podsumowanie

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Nuclei Isolation

Fresh Frozen Tumors

Single nucleus RNA seq

ATAC seq

Tissue Homogenization

Archival Frozen Tumors

Microscopy Verification

Filtration Process

Rozdziały w tym wideo

0:04

Introduction

1:01

Tissue Dissection and Dissociation

3:20

Gradient Centrifugation and Isolation of Nuclei

4:56

Results: Comparison of UMIs and Genes Obtained from Different Human Brain snRNA-seq Datasets

5:57

Conclusion

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