Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders
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6.1K Views
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10:28 min
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April 3rd, 2021
DOI :
April 3rd, 2021
•Camila F. Almeida1, Emma C. Frair1, Nianyuan Huang1, Reid Neinast2, Kim L. McBride2,3,4,6, Robert B. Weiss5, Kevin M. Flanigan1,6, Nicolas Wein1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University
Rozdziały w tym wideo
0:00
Introduction
0:36
Overview
1:23
Initiation of Demal Fibroblasts Culture
2:46
Establishment of FM Cell Lines Lentvirus-hTERT Transduction
3:57
Lentivirus-MyoD Transduction
4:54
Fibroblasts Transdifferentiation
6:11
Results
9:16
Conclusion
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