Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

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2.1K Views

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10:16 min

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January 20th, 2023

DOI :

10.3791/64360-v

January 20th, 2023

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Xinghui Song¹, Hao Shen², Yanwei Li³, Yueting Xing³, Jiajia Wang³, Chun Guo³, Yingying Huang³, Jin Chen²

¹Central Laboratory of Women’s Hospital, Zhejiang University School of Medicine, ²School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, ³Core Facilities, Zhejiang University School of Medicine

Podsumowanie

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Flow Cytometry

Sorting Parameters

Small Extracellular Vesicles

Optimization

High throughput Isolation

Fluorescent Particles

Quality Control QC

Sheath Fluid

Illumination Chamber

Rainbow Fluorescent Particles

Rozdziały w tym wideo

0:04

Introduction

0:34

Cell Sorting

5:40

Isolation of Small Extracellular Vesicles (sEV)

8:00

Results: Purification and Analysis of Small Extracellular Vesicles (sEV)

9:43

Conclusion

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