Cultivating Ex Vivo Patient-Derived Glioma Organoids Using a Tissue Chopper

1 So our research focuses on2 improving generation of patient derived organoids 3 for glioblastoma patients. 4 And our key questions here revolve around 5 whether this approach can efficiently 6 produce more organoids, reduce manufacturing time, 7 and at the same time maintain 8 the characteristics of the original tumor. 9 And we would use that for an effective drug 10 and immunotherapy screening in glioblastoma patients.

11 The recent developments in the glioblastoma field12 include advancement in understanding the genetic 13 and molecular aspects leading to 14 the recognition of new targets. 15 Additionally, there's a great growing emphasis 16 on using 3D organoids 17 to better replicate the complexity of tumors 18 and allow drug testing. 19 Currently, technologies like manual dissection, 20 enzymatic dissociation, organ-on-a-chip, 21 and bioprinting are being widely used to prepare organoids.

22 On the other hand, 23 mechanical chopping provides 24 metabolically active brain slices in various studies, 25 but lacks the methodological studies 26 for organoid preparation. 27 Our research has introduced an innovative method 28 for generating patient derived 3D organoids, 29 using an automated chopping machine, 30 we demonstrated a nearly 70%reduction in manufacturing time 31 and a significantly higher organoids count 32 compared to the manual dissection, 33 offering a more efficient method for drug 34 and immunotherapy screening in glioblastoma patients. 35 The advancement of our study significantly accelerates36 the organoid production process, 37 and at the same time is able 38 to maintain the main characteristics of the original tumors.

39 And by reducing the time as well as the resources required 40 for organoid generation, 41 our approach enhances the feasibility of large scale drug 42 and immunotherapy screening for glioblastoma patients.