Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

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08:07 min

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January 12th, 2024

DOI :

10.3791/66230-v

January 12th, 2024

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Maryam Sahi¹, Sarah Andersson¹, Cecilia Mattson¹, Matilda Dale¹, Sofia Kagiolglou¹, Camilla Hofström², Helena Persson², Jonas Klingström³^,⁴, Francesca Chiodi⁵, Claudia Fredolini¹

¹Affinity Proteomics-Stockholm Unit, SciLifeLab, Division of Affinity Proteomics, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, ²Human Antibody Therapeutics Unit, SciLifeLab, Division of Drug Discovery and Development, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, ³Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, ⁴Public Health Agency of Sweden, ⁵Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

Podsumowanie

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Surface Protein Epitopes

Viral Particles

Multiplex Dual reporter Strategy

Proteomic Analysis

Virus host Interaction

Antivirals

Vaccines

Mass Spectrometry

Immunoelectron Microscopy

Affinity Proteomics

SARS CoV 2

Membrane Proteins

High throughput Profiling

Magnetic Beads

Rozdziały w tym wideo

0:00

Introduction

1:25

Conjugation of ACE2 and Control Antibodies to Magnetic Microspheres to Capture Viral Particles from SARS-‐CoV‐2 Infected Cells