This work was funded by the Bureau of Science &#38; Technology Nanchong City [22JCYJPT0007].

The protocol follows the guidelines of the Human Research Ethics Committee of Nanchong Central Hospital.
1. Pre-operative workup
<ol>
	<li>Perform MRI scanning to confirm the diagnosis and assess the extent of the lesion, bile duct, and vascular anatomy. Perform a magnetic resonance cholangiopancreatography imaging scan on a 3.0 T MRI unit using T2 weighted image thick single-shot fast spin echo/turbo spin echo and fast acquisition relaxation enhancement sequences (Table of Materials).</li>
	<li>Perform the ICG retention test to effectively assess liver function.
	<ol>
		<li>Utilize the liver function reserve analyzer for experimental analysis. Input the patient&#39;s height, body weight, and hemoglobin concentration on the analyzer&#39;s touch screen. The software will automatically compute the required amount of ICG to be injected.</li>
		<li>Subsequently, rapidly inject the ICG through the left median cubital vein, while the nasal probe automatically measures the wavelength of the spectrophotometric spectra. Employ spectroscopic analysis to determine the concentration of ICG, followed by calculating the retention rate after a 10-min injection period8 (Table of Materials).</li>
	</ol>
	</li>
	<li>Extend invitations to surgical, anesthesiology, neurology, and cardiology experts for multidisciplinary consultation to develop surgical strategies, anesthesia management plans, and perioperative medication guidelines.</li>
</ol>
2. Anesthesia
<ol>
	<li>Administer pre-operative antibiotics, typically 1.0 g of ceftazidime (Table of Materials), intravenously on induction of anesthesia.</li>
	<li>Place an arterial line in the patient&#39;s left radial artery and insert a central venous line into the right internal jugular vein.</li>
	<li>Control the intraoperative central venous pressure (CVP) at 5-10 mmHg to ensure cerebral blood perfusion and avoid intraoperative hypotension.</li>
</ol>
3. Patient positioning
<ol>
	<li>Position the patient supine on the operating table in a split-leg position, with the camera assistant standing between the patient&#39;s legs, the first assistant on the patient&#39;s left side, and the surgeon on the patient&#39;s right side.</li>
	<li>Elevate the patient to 30&#176; right lateral position.</li>
</ol>
4. Port site insertion and laparoscope (Figure 2)
<ol>
	<li>Make a longitudinal incision 1 cm below the umbilicus and establish pneumoperitoneum using the Veress needle technique.</li>
	<li>Place 5 mm and 12 mm ports below the costal margin along the left and right anterior axillary lines.</li>
	<li>Place a 12 mm port below the rib cage along the midclavicular line on both the left and right sides.</li>
	<li>Maintain pneumoperitoneum pressure at 10-14 mmHg.</li>
	<li>Perform the procedure using a 30&#176; high-definition laparoscopic device (Table of Materials).</li>
</ol>
5. Operative steps
<ol>
	<li>Mobilization of the left lobe of the liver
	<ol>
		<li>Dissect the hepatic round ligament and falciform ligament using an ultrasonic scalpel (Table of Materials).</li>
		<li>Expose the root of the left hepatic vein. Completely divide the triangular ligament and coronary ligament to expose the root of the left hepatic vein.</li>
	</ol>
	</li>
	<li>Pringle&#39;s maneuver
	<ol>
		<li>Utilize laparoscopic Pringle&#39;s maneuver to apply an extracorporeal tourniquet, and initiate a first porta hepatis block with Pringle&#39;s maneuver if necessary9.</li>
		<li>Employ a grasper posterior to the hepatic pedicle via the foramen of Winslow to facilitate the placement of a cotton tape. Subsequently, extract the ends of the cotton tape through a 5 mm port trocar under the guidance of the grasper.</li>
		<li>Upon removal of the 5 mm trocar, thread one end of the cotton tape through a suction tube and advance into the abdominal cavity up to the level of the hepatic pedicle. Concurrently, maintain the external end of the cotton tape outside the patient&#39;s body.</li>
	</ol>
	</li>
	<li>Left lateral pedicle control via Glissonian approach
	<ol>
		<li>Dissect the superficial peritoneum with an ultrasonic scalpel along the left side of the LTH.</li>
		<li>Dissect the Glissonian pedicles for segments 2/3 from the ventral to the dorsal side.</li>
		<li>Excise the Glissonian pedicles for segments 2/3 with clips (Table of Materials) or a stapler (Table of Materials).</li>
	</ol>
	</li>
	<li>Parenchymal transection
	<ol>
		<li>Dissect the hepatic parenchyma ventrally along the left side of the falciform ligament with an ultrasonic scalpel.</li>
		<li>Use fine-tip forceps and induce energy stimulation to dissect liver parenchyma with an ultrasonic scalpel.</li>
		<li>Dissect the hepatic parenchyma along the UFV within the hepatic parenchyma, facilitating the identification of the root of the left hepatic vein.</li>
		<li>Divide small vessels with the ultrasonic scalpel.</li>
		<li>Divide large vessels or pedicle structures between clips.</li>
	</ol>
	</li>
	<li>Left hepatic vein transection
	<ol>
		<li>Transect the left hepatic vein with a stapler.</li>
	</ol>
	</li>
	<li>Bleeding control
	<ol>
		<li>Perform the first porta hepatis block with Pringle&#39;s maneuver if needed.</li>
		<li>Perform meticulous dissection and identification of intrahepatic vessels.</li>
		<li>Divide hilum and hepatic venules with clips or stapler.</li>
		<li>Use a vascular suture to secure bleeding from vessels.</li>
		<li>Utilize bipolar electrocoagulation forceps to coagulate hemorrhagic points.</li>
	</ol>
	</li>
</ol>
6. Specimen retrieval
<ol>
	<li>Place the specimen into a plastic bag and retrieve it through the subumbilical incision.</li>
</ol>

Laparoscopic Sectionectomy Using Ligamentum Teres for Bloodless Hepatectomy

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	<li>Liu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+hepatectomy+produces+better+outcomes+for+hepatolithiasis+than+open+hepatectomy:+An+updated+systematic+review+and+meta-analysis.">Laparoscopic hepatectomy produces better outcomes for hepatolithiasis than open hepatectomy: An updated systematic review and meta-analysis.</a> Int J Surg. 51, 151-163 (2018).</li><li>Abu Hilal, M., Pearce, N. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+left+lateral+liver+sectionectomy:+a+safe,+efficient,+reproducible+technique.">Laparoscopic left lateral liver sectionectomy: a safe, efficient, reproducible technique.</a> Dig Surg. 25 (4), 305-308 (2008).</li><li>Montorsi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perspectives+and+drawbacks+of+minimally+invasive+surgery+for+hepatocellular+carcinoma.">Perspectives and drawbacks of minimally invasive surgery for hepatocellular carcinoma.</a> Hepatogastroenterology. 49, 56-61 (2002).</li><li>Zhang, X. L., Wang, W. J., Wang, W. J., Cao, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effectiveness+and+safety+of+controlled+venous+pressure+in+liver+surgery:+a+systematic+review+and+network+meta-analysis.">Effectiveness and safety of controlled venous pressure in liver surgery: a systematic review and network meta-analysis.</a> Biomed Res Int. 2015, 290234 (2015).</li><li>Ogiso, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anatomy+of+the+middle+hepatic+vein+tributaries+to+promote+safer+hepatic+vein-guided+liver+resection.">Anatomy of the middle hepatic vein tributaries to promote safer hepatic vein-guided liver resection.</a> J Gastrointest Surg. 26 (1), 122-127 (2022).</li><li>Xie, K. L., Zeng, Y., Wu, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hepatic+trisectionectomy+for+hepatocellular+carcinoma+using+the+Glisson+pedicle+method+combined+with+anterior+approach.">Hepatic trisectionectomy for hepatocellular carcinoma using the Glisson pedicle method combined with anterior approach.</a> World J Surg. 38 (9), 2358-2362 (2014).</li><li>Sugioka, A., Kato, Y., Tanahashi, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+extrahepatic+Glissonean+pedicle+isolation+for+anatomical+liver+resection+based+on+Laennec's+capsule:+proposal+of+a+novel+comprehensive+surgical+anatomy+of+the+liver.">Systematic extrahepatic Glissonean pedicle isolation for anatomical liver resection based on Laennec's capsule: proposal of a novel comprehensive surgical anatomy of the liver.</a> J Hepatobiliary Pancreat Sci. 24 (1), 17-23 (2017).</li><li>Wu, P. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+assessment+of+liver+function+reserve+with+fluorescent+dosimetry+of+indocyanine+green.">Noninvasive assessment of liver function reserve with fluorescent dosimetry of indocyanine green.</a> Biomed Opt Express. 13 (4), 1995-2005 (2022).</li><li>Piardi, T., Lhuaire, M., Memeo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+Pringle+maneuver:+how+we+do+it.">Laparoscopic Pringle maneuver: how we do it.</a> Hepatobiliary Surg Nutr. 5 (4), 345-349 (2016).</li><li>Liu, T. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+controlled+low+central+venous+pressure+during+hepatectomy:+A+systematic+review+and+meta-analysis.">Application of controlled low central venous pressure during hepatectomy: A systematic review and meta-analysis.</a> J Clin Anesth. 75, 110467 (2021).</li><li>van der Poel, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+multicenter+propensity+score+matched+study+on+laparoscopic+versus+open+left+lateral+sectionectomy.">International multicenter propensity score matched study on laparoscopic versus open left lateral sectionectomy.</a> HPB (Oxford). 23 (5), 707-714 (2021).</li><li>Darnis, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+abdominal+wall+benefits+of+the+laparoscopic+approach+in+liver+left+lateral+sectionectomy:+a+multicenter+comparative+study.">Long-term abdominal wall benefits of the laparoscopic approach in liver left lateral sectionectomy: a multicenter comparative study.</a> Surg Endosc. 35 (9), 5034-5042 (2021).</li><li>Chong, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+robotic+and+laparoscopic+liver+resection+study+group+investigators.+An+international+multicentre+propensity+score+matched+analysis+comparing+between+robotic+versus+laparoscopic+left+lateral+sectionectomy.">International robotic and laparoscopic liver resection study group investigators. An international multicentre propensity score matched analysis comparing between robotic versus laparoscopic left lateral sectionectomy.</a> Surg Endosc. 37 (5), 3439-3448 (2023).</li><li>Zheng, K., He, D., Liao, A., Wu, H., Yang, J., Jiang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+segmentectomy+IV+using+hepatic+round+ligament+approach+combined+with+fluorescent+negative+staining+method.">Laparoscopic segmentectomy IV using hepatic round ligament approach combined with fluorescent negative staining method.</a> Ann Surg Oncol. 29 (5), 2980-2981 (2022).</li><li>Wu, H., Xie, K. L., Huang, J., Pan, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+effect+of+fissure+for+ligamentum+teres+hepatic+approach+in+hepatectomy.">Clinical effect of fissure for ligamentum teres hepatic approach in hepatectomy.</a> Chin J Dig Surg. 15 (1), 53-57 (2016).</li><li>Idrees, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Umbilical+fissure+vein,+anatomical+variation+and+potential+surgical+application.">Umbilical fissure vein, anatomical variation and potential surgical application.</a> ANZ J Surg. 91 (7-8), E479-E483 (2021).</li><li>Kobayashi, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extended+segmentectomy+II+to+left+hepatic+vein:+Importance+of+preserving+umbilical+fissure+vein+to+avoid+congestion+of+segment+III.">Extended segmentectomy II to left hepatic vein: Importance of preserving umbilical fissure vein to avoid congestion of segment III.</a> J Am Coll Surg. 225 (3), e5-e11 (2017).</li><li>Zheng, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laparoscopic+anatomic+bi-segmentectomy+(S3+and+S4b)+using+the+Glisson's+pedicle-first+and+intrahepatic+anatomic+markers+approach.">Laparoscopic anatomic bi-segmentectomy (S3 and S4b) using the Glisson's pedicle-first and intrahepatic anatomic markers approach.</a> Surg Endosc. 36 (10), 7859-7860 (2022).</li><li>Hobeika, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+cirrhosis+in+patients+undergoing+laparoscopic+liver+resection+in+a+nationwide+multicentre+survey.">Impact of cirrhosis in patients undergoing laparoscopic liver resection in a nationwide multicentre survey.</a> Br J Surg. 107 (3), 268-277 (2020).</li></ol>

The authors declare no competing interests.

Management of intraoperative bleeding remains a crucial challenge in laparoscopic hepatectomy. To address this issue, Pringle&#39;s maneuver and the CLCVP technique are commonly employed to control hepatic blood flow10. However, not all patients are suitable candidates for CLCVP, particularly those with concurrent cardiovascular and cerebrovascular diseases.
In this study, we present our experience with laparoscopic hepatectomy in patients with comorbid cerebrovascular ...

Laparoscopic techniques are extensively used in liver surgery and are considered safe and effective. Compared with open surgery, laparoscopic left lateral sectionectomy (LLLS) offers several advantages, including a reduced overall complication rate, shorter postoperative hospitalization, and decreased blood loss1. In the traditional LLLS procedure, the parenchyma is dissected using a harmonic scalpel, starting on the upper liver surface, proceeding from front to back, taking layers 2-3 mm deep ventral and dorsal to the level of the LHV, followed by direct dissection of the left outer lobe hepatic hilum with a staple2. This method may lead to injury of the hepatic parenchyma and veins, thereby increasing the risk of bleeding. The controlled low central venous pressure (CLCVP) technique is often employed in traditional LLLS procedures to mitigate intraoperative bleeding3. However, in patients with concurrent cardiovascular and cerebrovascular diseases, anesthesiologists may opt to avoid using the CLCVP technique to prioritize the perfusion of vital organs4. Herein, we present a standardized procedure for LLLS that does not rely on the CLCVP technique but effectively manages intraoperative bleeding. The key components of the procedure are as follows: (1) utilizing the ligamentum teres hepatis (LTH) approach to control the Glissonian pedicles for segments 2/3; (2) determining the transection plane of the liver based on anatomical landmarks such as the falciform ligament, umbilical fissure vein (UFV), and the canal of Arantius; and (3) using the UFV as a guide for hepatic parenchymal transection to enhance accessibility to the root of the left hepatic vein. The rationale for this technique, which is guided by anatomical landmarks such as the Glissonian pedicle and intrahepatic veins (e.g., UFV), enables more precise lobular and segmental resections while reducing the risk of hemorrhage5. This procedure is straightforward and easy to disseminate and learn. Xie et al.6 and Prof. Sugioka et al.7 have highlighted the safety, effectiveness, simplicity, and anatomical correctness of the LTH approach in hepatectomy procedures. We introduced the LLLS procedure guided by LTH and UFV to further improve the surgical technique.
In this study, we present a representative case to elucidate the procedural steps involved. A 74-year-old male patient presented with chronic upper abdominal pain that had persisted for 3 months. Pre-operative magnetic resonance imaging (MRI) revealed left intrahepatic bile duct stones and localized cystic dilatation of the bile ducts (Figure 1A,B). Additionally, the patient had a history of two cerebral infarctions. Head MRI indicated multiple ischemic and infarct lesions in various brain regions, including the bilateral periventricular regions, basal ganglia, corona radiata, brainstem, and frontal lobes. Notably, lesions in the left basal ganglia and adjacent parts of the right lateral ventricle softened with gliosis. The patient&#39;s Child-Pugh score was 5 (Grade A), and indocyanine green (ICG) retention at 15 min was 6.5%. Based on the radiological features, the patient was diagnosed with left intrahepatic bile duct stones. Subsequently, the patient underwent LLLS.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>30&#176; high-definition laparoscopic device</td>
			<td>KARL STORZ,Germany</td>
			<td>26606BCA/ACA</td>
			<td>New 3D Electronic Laparoscope</td>
		</tr>
		<tr>
			<td>Bipolar electrocoagulation</td>
			<td>KANGJI, China</td>
			<td>KJ-XRH05Q</td>
			<td>Electrocoagulation for hemostasis</td>
		</tr>
		<tr>
			<td>Ceftazidime</td>
			<td>Zhejiang Jutai Pharmaceutical Co.,China</td>
			<td>(China) Drug Administration Code (DAC)H20033369</td>
			<td>usage: 1.0 g, intravenous drip</td>
		</tr>
		<tr>
			<td>Computed Tomography (CT)</td>
			<td>Siemens, Germany</td>
			<td>SOMATOM Force</td>
			<td>Force is a 96-row dual-source CT scanner that revolutionizes a series of imaging chains including the tube, high-voltage generator, detector, data acquisition system, and reconstruction system, opening up a new era of CT imaging and achieving faster, wider, thinner, more capable, and lower radiation dose.</td>
		</tr>
		<tr>
			<td>Echelon Flex Endopath Stapler</td>
			<td>Ethicon</td>
			<td>EC60A</td>
			<td>Manual stapler that compresses 
			tissue while it simultaneously lays 
			down a staple line and transects the 
			tissue, 60 mm Stapler (Standard), Size 60 mm, Length 34 cm</td>
		</tr>
		<tr>
			<td>Harmonic ACE+7 Shears</td>
			<td>Ethicon</td>
			<td>HARH36</td>
			<td>Curved tip, energy sealing and 
			dissecting, diameter 5 mm, length 36 cm</td>
		</tr>
		<tr>
			<td>Hem-o-lok Clips L</td>
			<td>Weck Surgical Instruments, Teleflex Medical, Durham, NC</td>
			<td>544240</td>
			<td>Vascular clip 5&#8211;13 mm Size Range</td>
		</tr>
		<tr>
			<td>Hem-o-lok Clips ML</td>
			<td>Weck Surgical Instruments, Teleflex 
			Medical, Durham, NC</td>
			<td>544230</td>
			<td>Vascular clip 3&#8211;10 mm Size Range</td>
		</tr>
		<tr>
			<td>Indocyanine Green(ICG)</td>
			<td>&#160;Dandong Medical Creation Pharmaceutical Co., Ltd.</td>
			<td>H20055881</td>
			<td>25 mg/vial, Detecting liver reserve function</td>
		</tr>
		<tr>
			<td>Liver function reserve analyzer</td>
			<td>Shanghai Optoelectronic Medical Electronic Instruments Co., Ltd</td>
			<td>DDG3300K</td>
			<td>A medical instrument that detects and analyzes indocyanine green (ICG) injected into the body based on spectroscopic analysis techniques.</td>
		</tr>
		<tr>
			<td>Magnetic resonance imaging (MRI)</td>
			<td>GE company,American</td>
			<td>Signa Hoxt 3.0T MRI,JB00988XC</td>
			<td>provides 360-degrees of coil coverage, RF technology, and a direct digital interface with more channels. Patient-friendly design maximizes comfort and system utility, accommodating all types of patients and sizes with feet-first imaging.</td>
		</tr>
	</tbody>
</table>

laparoscopic left lateral sectionectomy: guided by the ligamentum teres hepatis and the umbilical fissure vein

Laparoscopic left lateral sectionectomy (LLLS), a mainstream procedure in liver surgery, often utilizes controlled low central venous pressure (CLCVP) to reduce bleeding in the hepatic venous system. However, anesthesiologists may avoid the use of CLCVP in patients with concurrent cardiovascular and cerebrovascular diseases to prioritize the maintenance of vital organ perfusion. In this report, we present an LLLS guided by the ligamentum teres hepatis (LTH) for dissection of the Glissonian pedicles for segments 2/3 outside the liver, followed by hepatic parenchymal dissection along the falciform ligament and umbilical fissure vein (UFV) while approaching the left hepatic vein root. Guided by LTH and UFV, this LLLS procedure effectively controlled intraoperative bleeding, even in the absence of CLCVP. Additionally, hepatectomy guided by extrahepatic and intrahepatic anatomical landmarks prevents loss of direction during liver dissection and ensures precise hepatic resection. These attributes suggest that the potential benefits extend beyond patients with cardiovascular or cerebrovascular conditions, making it applicable in a wide range of LLLS cases.

In the representative case, the operative time was 120 min with an estimated blood loss of 50 mL, and there were no postoperative complications. The postoperative hospital stay was 7 days. Table 1 summarizes the intra- and postoperative data. A computed tomography(CT) performed on postoperative day 5 revealed no evidence of blood or fluid accumulation in the liver section (Figure 3). The patient was successfully discharged from hospital after surgery. Histological examinatio...

Author Spotlight: Advancing Hepatobiliary and Pancreatic Tumor Treatment with Minimally Invasive Surgical Techniques

Laparoscopic left lateral sectionectomy guided by the ligamentum teres hepatis and umbilical fissure vein effectively controls intraoperative bleeding even without controlled low central venous pressure and prevents loss of direction during parenchymal dissection.

Laparoscopic Left Lateral Sectionectomy: Guided by the Ligamentum Teres Hepatis and the Umbilical Fissure Vein

Laparoscopic left lateral sectionectomy (LLLS), a mainstream procedure in liver surgery, often utilizes controlled low central venous pressure (CLCVP) to ...

laparoscopic-left-lateral-sectionectomy-guided-ligamentum-teres

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488 Views.  the Affiliated Nanchong Central Hospital of North Sichuan Medical College (University). Laparoscopic left lateral sectionectomy guided by the ligamentum teres hepatis and umbilical fissure vein effectively controls intraoperative bleeding even without controlled low central venous pressure and prevents loss of direction during parenchymal dissection.

Video: Author Spotlight: Advancing Hepatobiliary and Pancreatic Tumor Treatment with Minimally Invasive Surgical Techniques

Nerve Stimulator-guided Injection of Autologous Stem Cells Near the Equine Left Recurrent Laryngeal Nerve

Recurrent laryngeal neuropathy (RLN) commonly affects horses and is characterized by abnormal respiratory sounds and exercise intolerance. The recurrent laryngeal nerve shows lesions of demyelination. The benefit of applying stem cells to demyelinated nerves has been demonstrated in various animal models. The aim of the study was to test the feasibility and safety of a peri-neuronal injection of autologous muscle-derived mesenchymal stem cells to the left recurrent laryngeal nerve in healthy horses by using an electrical nerve stimulator.
Muscle-derived stems cell are obtained from five healthy Standardbred horses by sampling 20 mg of muscle tissue with a semi-automatic 14 G biopsy needle from the triceps muscle. Movements of the larynx are monitored via upper-airway video endoscopy. The left recurrent laryngeal nerve is approached with an insulated nerve block needle. Nerve stimulation is applied, starting at 2 mA, and the successful abduction of the left arytenoid is monitored. The stimulation intensity is reduced progressively. When a loss of the motor response is observed at 0.5 mA, 107 autologous muscle-derived stem cells are injected. Two examiners, who are blinded to the time point, score the laryngeal function of the horses prior to the treatment and at day 1, day 7, and day 28 after the injection of the cells. In a sixth horse, 1 mL of 2% lidocaine is injected to further confirm the correct positioning of the needle. This leads to a temporary paralysis of the left arytenoid cartilage.
This study proves that the recurrent laryngeal nerve can be approached with the help of an electrical nerve stimulator and that the electrical stimulation of the nerve is well tolerated by the horses. No modification of the laryngeal function was observed in any of the horses after the injection of the stem cells. Further studies should be conducted to describe the effects of a peri-neuronal injection of autologous muscle-derived mesenchymal stem cells to horses suffering from RLN.

Here, we present a protocol to inject autologous muscle-derived stem cells in the proximity to the recurrent laryngeal nerve with the help of an electrical nerve stimulator. This novel technique may become useful for the treatment of equine recurrent laryngeal neuropathy.

Recurrent laryngeal neuropathy (RLN) commonly affects horses and is characterized by abnormal respiratory sounds and exercise intolerance. The recurrent ...

Standardized Hemorrhagic Shock Induction Guided by Cerebral Oximetry and Extended Hemodynamic Monitoring in Pigs

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an insufficient oxygen supply and irreversible organ failure. The brain exerts only limited compensation capacities and is particularly at high risk of severe hypoxic damage.This article demonstrates the reproducible induction of life-threatening hemorrhagic shock in a porcine model by means of calculated blood withdrawal. We titrate shock induction guided by near-infrared spectroscopy&#160;and extended hemodynamic monitoring to display systemic circulatory failure, as well as cerebral microcirculatory oxygen depletion. In comparison to similar models that primarily focus on predefined removal volumes for shock induction, this approach highlights a titration by means of the resulting failure of macro- and microcirculation.

Hemorrhagic shock is a severe complication in seriously injured patients, which leads to life-threatening oxygen undersupply. We present a standardized method to induce hemorrhagic shock via blood withdrawal in pigs that is guided by hemodynamics and microcirculatory cerebral oxygenation.

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an ...

Laparoscopic Radical Left Pancreatectomy for Pancreatic Cancer: Surgical Strategy and Technique Video

Radical resection margins, resection of Gerota&#8217;s (perirenal) fascia, and adequate lymph node dissection are crucial for an adequate oncological resection of left-sided pancreatic cancer. Several surgical techniques have been described in recent years, but few were specifically designed for minimally invasive approaches. This study describes and demonstrates a standardized and reproducible technique for an adequate oncological resection of pancreatic cancer: laparoscopic radical left pancreatectomy (LRLP).
A 61-year-old woman presented with an incidental finding of a 3 cm mass in the left pancreas suspect for malignancy. Imaging did not reveal distant metastases, central vascular involvement, or morbid obesity, hence the patient was suitable for LRLP. This study describes the main steps of LRLP for pancreatic cancer. First, the lesser sac is opened by transecting the gastrocolic ligament. The splenic flexure of the colon is mobilized and the inferior border of the pancreas including Gerota&#39;s fascia is dissected down to the inferior border of the spleen. The pancreas is tunneled and hung, including Gerota&#8217;s fascia with a vessel loop. At the pancreatic neck, a tunnel is created between the pancreas and the portal vein, likewise a vessel loop is passed. The pancreas is then transected using the graded compression technique with an endostapler. Both the splenic vein and artery are transected before completing the resection. The entire specimen is extracted in a retrieval bag via a small Pfannenstiel incision.
Duration of the surgery was 210 min with 250 mL blood loss. Pathology revealed a R0-resection (&#62;1 mm) of a well-to-moderately differentiated adenocarcinoma originating from an intraductal papillary mucinous neoplasm. A total of 15 tumor-negative lymph nodes were resected. This is a detailed description of LRLP for left-sided pancreatic cancer as is currently being used within the international, multicenter randomized DIPLOMA (Distal Pancreatectomy Minimally Invasive or Open for PDAC) trial.

Oncologically safe left pancreatectomy requires radical resection (R0), Gerota&#8217;s (perirenal) fascia resection, and adequate lymph node dissection. This study describes the technical details of laparoscopic radical left pancreatectomy (LRLP), used in the first international multicenter randomized trial comparing minimally invasive with open left pancreatectomy for pancreatic cancer, the DIPLOMA trial.

Radical resection margins, resection of Gerota&#8217;s (perirenal) fascia, and adequate lymph node dissection are crucial for an adequate oncological ...

Laparoscopic Non-Mesh Cerclage Pectopexy for Pelvic Organ Prolapse

Pelvic organ prolapse (POP) is widespread among the female population and significantly impairs the patient's quality of life. It is important to restore apical support for treating POP. Sacrocolpopexy and pectopexy are indicated for apical prolapse. Using a synthetic mesh in these techniques increases success by enhancing apical support. However, the implantation of synthetic mesh is associated with mesh-related complications. In addition, the exorbitant cost of synthetic mesh and lack of universal access limit the popularity of these procedures. The current study develops a unique technique known as laparoscopic non-mesh cerclage pectopexy (LNMCP), in which permanent cervical cerclage sutures are embedded in the round ligament until the iliopectineal ligament. The iliopectineal ligament was sutured, resulting in a firm cervical suspension. The procedure was successfully performed in 16 cases in the hospital. The surgical duration was 67.8 min &plusmn; 15.5 min, and the blood loss was 73.1 mL &plusmn; 51.1 mL. No procedural complications were seen. LNMCP is associated with an objective success rate of 100% and a subjective success rate of 93.8%. LNMCP for patients with apical prolapse obviates the need for a mesh, thereby avoiding complications associated with mesh erosion and reducing medical costs. In addition, it is easy to perform even in resource-poor areas without access to synthetic mesh.

The present pilot study describes the development of laparoscopic non-mesh cerclage pectopexy to treat pelvic organ prolapse. The procedure can be used to prevent any complications associated with the use of mesh.

Pelvic organ prolapse (POP) is widespread among the female population and significantly impairs the patient's quality of life. It is important to restore ...

ICE-Based 3D Remodeling for the Left Atrium and Pulmonary Vein

Three-Dimensional Modeling of the Left Atrium and Pulmonary Veins with a Precise Intracardiac Echocardiography Approach

Intracardiac echocardiography (ICE) is a novel tool for estimating cardiac anatomy during pulmonary vein isolation procedures, particularly the left atrium (LA) anatomy and pulmonary vein structures. ICE is widely used to establish a three-dimensional (3D) left atrial structural model during ablation procedures. However, it is unclear whether using ICE in a precise 3D modeling method can provide a more accurate left atrial 3D model and the transseptal approach. This study proposes a protocol to model the left atrium and pulmonary veins with ICE and fast anatomical mapping (FAM) catheter remodeling. It evaluates the accuracy of the models produced using the two methods through observer scoring. We included 50 patients who underwent ICE-based 3D remodeling and 45 who underwent FAM 3D remodeling based on pulmonary vein isolation procedures. The pulmonary vein antrum remodeling is estimated by comparing the antrum area acquired by remodeling and left atrial computed tomography angiography (CTA). The observer scores for the modeling in the ICE and FAM groups were 3.40 &#177; 0.81 and 3.02 &#177; 0.72 (P &#60; 0.05), respectively. The pulmonary vein antrum area obtained using the ICE- and FAM-based methods showed a correlation with the area acquired by left atrium CT. However, the 95% confidence interval bias was narrower in ICE-acquired models than in FAM-acquired models (-238 cm2 to 323 cm2 Vs. -363 cm2 to 386 cm2, respectively) using Bland-Altman analysis. Therefore, precise ICE possesses high accuracy in estimating the left atrial structure, becoming a promising approach for future cardiac structure estimation.

Author Spotlight: Advancements in Intracardiac Echocardiography for Atrial Anatomy Assessment 

Accurate intracardiac echocardiography (ICE) shows significant accuracy in estimating the left atrial structure, a prospective and promising cardiac structure estimation method. Here we propose a protocol for three-dimensional modeling of the left atrium and pulmonary veins with ICE and fast anatomical mapping (FAM) catheter remodeling.

Intracardiac echocardiography (ICE) is a novel tool for estimating cardiac anatomy during pulmonary vein isolation procedures, particularly the left ...

Advancing LRPS: Optimal Techniques for Challenging Liver Resections

Techniques of Laparoscopic Right Posterior Sectionectomy: Glissonian Approach and a Parenchymal Transection Technique

Laparoscopic liver resections (LLR) have been widely accepted as a treatment option for liver tumors. They offer several advantages over open liver resections, including less blood loss, reduced wound pain, and shorter hospital stays with a comparable oncological outcome. However, laparoscopic resection of lesions in the right posterior section of the liver is challenging due to difficulties in bleeding control and visualizing the surgical field. In the past, laparoscopic right posterior sectionectomy (LRPS) was still in the exploration phase, with undefined risks in the Second International Consensus Conference on LLR in 2014. However, recent technological advancements and increased surgical experience have shown that LRPS can be safe and feasible. It has been found to reduce hospital stay and blood loss compared to open surgery. This manuscript aims to provide a detailed description of the steps involved in LRPS. The key factors contributing to our success in this challenging procedure include proper liver retraction and exposure, the use of an intrahepatic Glissonian approach for inflow control, a technique called the 'ultrasonic scalpel mimic Cavitron ultrasonic surgical aspirator (CUSA)' for parenchymal transection, early identification of the right hepatic vein, and meticulous bleeding control using bipolar diathermy.

Here, we present a protocol to perform laparoscopic right posterior sectionectomy, focusing on two key aspects: the intrahepatic Glissonian approach for inflow control and a parenchymal transection technique using an ultrasonic surgical aspirator.

Laparoscopic liver resections (LLR) have been widely accepted as a treatment option for liver tumors. They offer several advantages over open liver ...

Laparoscopic Posterior Radical Antegrade Modular Pancreatosplenectomy for Distal Pancreatic Carcinoma

Distal pancreatic carcinoma is a highly malignant tumor with strong invasiveness, often growing to the edge of the pancreas and penetrating the pancreatic capsule to infiltrate surrounding tissues. In conventional distal pancreatosplenectomy (DPS), tumor cells are prone to spread along the direction of blood and lymphatic reflux due to surgical compression. Additionally, inflammation makes it challenging to achieve R0 resection, leading to a lower patient survival rate. To address these limitations, radical antegrade modular pancreatosplenectomy (RAMPS) was developed, emphasizing deeper excision, including the left anterior renal fascia, the left anterior renal adipose sac, and even the left adrenal gland, to improve the R0 resection rate. With the advancement of minimally invasive surgical techniques, laparoscopic RAMPS (L-RAMPS) is being considered technically safe and feasible in oncology. However, due to technical difficulties and a lack of supporting evidence for clinical application, only a few institutions are currently conducting L-RAMPS. In this context, this article presents detailed techniques for laparoscopic posterior radical antegrade modular pancreatosplenectomy (L-pRAMPS), offering promise for future clinical applications.

Laparoscopic radical antegrade modular pancreatosplenectomy (L-RAMPS) is currently considered technically safe and feasible. However, due to technical challenges and a lack of supporting evidence for widespread clinical applications, only a limited number of institutions are currently conducting L-RAMPS. This article aims to provide detailed techniques for laparoscopic posterior radical antegrade modular pancreatosplenectomy.

Distal pancreatic carcinoma is a highly malignant tumor with strong invasiveness, often growing to the edge of the pancreas and penetrating the pancreatic ...

Studying Liver Regeneration of ALLPS in Animal Models for Staged Hepatectomy

A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative mortality, severely impacting patient outcomes. In a robust hepatic environment, the future liver remnant (FLR) must exceed 25%, and in cases of cirrhosis, this requirement increases to over 40%. The inadequacy of FLR is currently a major obstacle in the progression of hepatic surgery.
Traditional methods to enhance FLR hypertrophy mainly focus on portal vein embolization (PVE), but its effectiveness is considerably limited. In recent years, there have been numerous reports on a novel biphasic hepatectomy method involving hepatic partitioning and portal vein ligation, known as associating liver partition and portal vein ligation for staged hepatectomy (ALPPS). ALPPS surpasses PVE in efficiently and considerably inducing FLR hypertrophy. However, the detailed mechanisms driving ALPPS-facilitated hepatic regeneration are not fully understood. Thus, replicating ALPPS in animal models is crucial to thoroughly investigate the molecular mechanisms of hepatic regeneration, offering valuable theoretical and practical insights.

Author Spotlight: Advancing Liver Regeneration Research through ALPPS Mouse Model

Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) has been shown to result in remarkable liver regeneration after several weeks post first-stage surgery. Animal models that have undergone ALPPS have been developed to explore potential liver regeneration ability and achieve favorable clinical outcomes.

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative ...

AAV-Based Therapies via Intravenous Injection in Preclinical Mouse Models

Consistent Delivery of Adeno-Associated Virus via Lateral Tail-Vein Injection in Adult Mice

Many disorders affect multiple organs or involve different regions of the body, so it is critical to deliver therapeutics systemically to target the affected cells located in different sites. Intravenous injection is a widely used systemic delivery route in preclinical studies that assess treatments intended for body-wide administration. In adult mice, it involves the intravenous administration of the therapeutic agent into the mouse&#39;s lateral tail veins. When mastered, tail-vein injections are safe and fast, and only require simple and commonly available tools. However, tail-vein injections are technically challenging and require extensive training and continuous practice to ensure the accurate delivery of the intended dose.
Here we describe a detailed, optimized, lateral tail-vein injection protocol that we have developed based on our experience and on recommendations that had been previously reported by other groups. Other than the mouse restrainers and insulin syringes, this protocol requires only reagents and equipment that are readily available in most labs. We found that following this protocol results in consistently successful intravenous delivery of adeno-associated virus (AAV) into the tail veins of unsedated 7-9 week-old mice. Additionally, we describe the optimized protocols for the histological detection of fluorescent reporter proteins and vector genome per diploid genome (vg/dg) quantification used to assess AAV transduction and biodistribution. The goal of this protocol is to aid experimenters in easily performing tail-vein injections successfully and consistently, which can reduce the practice time needed to master the technique.

Author Spotlight: Developing Gene Therapies for Muscular Dystrophies and Neuromuscular Disorders

Here we detail an optimized protocol for mouse lateral tail-vein injection to systemically administer adeno-associated virus (AAV) in adult mice. Additionally, we describe protocols of commonly used assays to assess AAV transduction.

Many disorders affect multiple organs or involve different regions of the body, so it is critical to deliver therapeutics systemically to target the ...