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Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

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11:32 min

October 18th, 2024

DOI :

10.3791/67258-v

October 18th, 2024


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Real time Microscopy

Rozdziały w tym wideo

0:00

Introduction

1:48

Neutrophil Isolation from Human Peripheral Blood for Assessing Pharmacology of NETosis Antagonists

4:25

Optimized Assay for NETosis Analysis Using Multiple 96‐Well Imaging Plates with Stimuli and Antagonists

5:37

Configuring Live Cell Microscopy Analysis System Software for the Phase Contrast and Immunofluorescence Imaging of Neutrophils

7:27

Analyzing Neutrophil Extracellular Trap (NET) Formation Using Live Imaging Microscopy Software

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