Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells
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07:37 min
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January 31st, 2025
DOI :
January 31st, 2025
•1T Cell Engineering, Mayo Clinic, 2Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, 3Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 4Center for Regenerative Biotherapeutics, Mayo Clinic, 5Division of Hematology, Mayo Clinic, 6Department of Immunology, Mayo Clinic
Rozdziały w tym wideo
0:00
Introduction
1:57
CART Cell Restimulation and Next Generation Sequencing Sample Preparation During CRISPR gRNA Selection
5:22
Preparation of Genomic DNA from CART Cells for CRISPR Screen Sequencing
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