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Neutrophil Isolation Protocol

101.8K Views

07:12 min

July 23rd, 2008

DOI :

10.3791/745-v

July 23rd, 2008


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Neutrophil Isolation

Rozdziały w tym wideo

0:00

Introduction

0:08

Equipment

0:24

Materials

1:24

Preparation of HBSS/HSA

2:06

Blood sample

2:38

Stages of neutrophil isolation

2:53

First separation: acquire neutrophils

4:56

Second separation: refine neutrophil layer

5:39

Third separation: lysing the red blood cells

6:27

Fourth separation: resuspending the neutrophils

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