All experiments in this protocol were approved by the Veterinary Authorities of the Jiangxi Provincial People&#39;s Hospital (number 70/2022). Furthermore, all experimental steps were performed in strict compliance with the Institutional Animal Care and Use Committee.
1. Start of surgery
NOTE: Male C57BL/6 mice weighing 20-22 g were housed in a well-ventilated cage, maintained in a standard pathogen-free environment with a 12 h/12 h light/dark cycle. The animals were provided ad libitum access to food and water at a controlled ambient temperature of 22 &#177; 1 &#176;C. The surgery was performed in a dedicated microsurgical room using a surgical microscope (Figure 1). The mice in the sham group were only operated by laparotomy, but no operation was performed on the liver. In the PVL group, part of the portal vein branches was ligated, and in the ALPPS group, the middle liver lobe was transected on the basis of portal vein ligation (PVL).
<ol>
	<li>Subject the mice to a 4-6 h&#160;preoperative fasting period. 
	NOTE: Fasting is required because eating causes gastric bulge in mice, it results in poor surgical field and is prone to accidental damage to the gastric wall.</li>
	<li>Arrange sterile surgical instruments, including microforceps, scissors, abdominal retractors, and pre-prepared 8-0 portal vein ligature sutures, on a side table. Soak intraoperative materials comprising sterile gauze and cotton balls in 37 &#176;C saline solution.</li>
	<li>For induction, expose the animals to 5% v/v isoflurane for 30 s, followed by maintenance anesthesia using 3 vol% isoflurane until achieving a deep anesthetic state. Examine the toe pinch reflex to ascertain the level of anesthesia. Subcutaneously inject medetomidine at a dose of 5 mg/kg during the induction phase.</li>
	<li>Apply ointment to the animals&#39; eyes and inject 1 mL of lactated Ringer&#39;s solution mixed with 5% glucose subcutaneously, dividing the dose equally between the lower abdomen on each side.</li>
	<li>Meticulously shave the abdominal region using small animal hair clippers, encompassing a 2 cm lateral area from the xiphoid process to the genital region.</li>
	<li>Thoroughly disinfect the surgical site 3 times&#160;with alternating rounds of povidone-iodine and alcohol.</li>
	<li>Make a length of 2.5-3.0 cm midline incision on the skin using a surgical scalpel, subsequently utilizing surgical scissors to delicately open the abdominal cavity.</li>
	<li>Employ the abdominal retractor to expand the incision, facilitating the gentle traction of the duodenum and a segment of the small intestine using sterile, moist cotton swabs. Cover these structures with the pre-prepared sterile gauze, simultaneously exposing the hepatic segment of the portal vein.</li>
</ol>
2. Portal vein ligation (PVL)
NOTE: Ensure that all actions are gentle throughout the whole procedure. Use moistened cotton balls when manipulating liver tissue.
<ol>
	<li>Peel off the peritoneum and intestinal tract and make sure the portal vein is fully exposed.</li>
	<li>In the course of exposing the main portal vein, proceed by encircling its branches using slow, deliberate forward-pushing and spreading movements.</li>
	<li>Sequentially identify the various branches of the portal vein in the following order: (1) the right posterior branch, (2) the combined left lateral and left median branches, and (3) the caudate branch (Figure 2A).</li>
</ol>
3. Dissection of the right posterior branch of portal vein branches
<ol>
	<li>Upon traversing the hepatic hilum via the main portal vein, observe a conspicuous branch of the right posterior lobe that has become visible.</li>
	<li>Use moistened cotton balls as a fulling agent and separate the liver&#39;s middle lobe and the right posterior lobe to avoid potential injury.</li>
	<li>Be sure to dissect along the Glisson&#39;s capsule (the peritoneum that extends over the surface of the liver) and pull a 1.5 cm silk to tie around portal vein branches.</li>
	<li>Ligate the right posterior branch of the portal vein with 8-0 silk suture. A reliable indicator of successful portal vein ligation is visible pallor of the liver.</li>
</ol>
4. Dissection of the left lateral and left medial branches of the portal vein
<ol>
	<li>The left lateral lobe (LLL) and left medial lobe (LML) branches share a common main portal vein. Be sure to dissect any potential interstice between the LLL and the caudate lobe. 
	NOTE: Ligation of the LLL and LML branches of the portal vein is the most difficult in this surgical model because the microforceps must traverse the space around the portal vein and avoid liver injury.</li>
	<li>Introduce the microforceps through the entrance in step 4.1 and move the hand slowly, as even the slightest resistance hints at the potential proximity to the liver or blood vessels.</li>
	<li>When resistance is felt, adjust the microforceps slightly to move forward and through the surface of Glisson&#39;s capsule of the right medial lobe (RML) (Figure 3C).</li>
	<li>Employ the prepared 8-0 silk suture to tie around the portal vein of the LLL and LML branches. Look for a striking ischemic demarcation within the liver middle lobe, while the left lobe assumes pallor, upon ligation of the portal vein branches.</li>
</ol>
5. Dissection of the caudate branch of the portal vein branches
<ol>
	<li>Dissect the caudate lobe and use a cotton swab to fill the potential space between the main portal vein and the caudate lobe.</li>
	<li>Peel off the peritoneum of the caudate lobes to visualize the portal vein branches of the lobe.</li>
	<li>Use Stitch-up suture rather than tie-around to ligate the caudate branch, ensuring that the needle path adequately wraps the caudate branch of the portal vein. 
	NOTE: The difficulty of ligating the caudate branch of the portal vein is because the caudate lobe portal vein encapsulates the liver tissue and is close to the main portal vein. A needle penetration depth of 1 mm is recommended.</li>
</ol>
6. Liver transection and cholecystectomy
<ol>
	<li>Observe a distinct demarcation line in the middle liver lobe and perform the transection along this line (Figure 2B).</li>
	<li>Use an electrocautery pen to create a 0.5 mm precut line along the demarcation line.</li>
	<li>Use micro forceps and an electrocautery pen to cut liver tissue, applying cotton swabs to stop bleeding. 
	NOTE: The difficulty in the transection is to master the depth. The goal is to split the liver as close to the vena cava as possible. Intraoperative bleeding is difficult to avoid and requires repetitive practice to master the depth of transection. Hot burning, cotton swabs, and gelatin sponges can be used to assist in hemostasis.</li>
	<li>Remove the gallbladder last as this will provide a good pulling point during the whole operation.</li>
	<li>Employ a cotton swab to apply controlled pressure for hemostasis, and if hemorrhage persists, the inferior vena cava is injured and the effect of suture repair will be poor; euthanize the animal without hesitation.</li>
</ol>
7. Final steps of surgery
<ol>
	<li>Ensure meticulous placement of the small bowel within the abdominal cavity prior to closure.</li>
	<li>Securely close the peritoneum and abdominal wall using 6-0 absorbable sutures and the skin with 4-0 absorbable sutures.</li>
	<li>Disinfect the skin surface again to ensure optimal cleanliness.</li>
	<li>Subcutaneously administer 1 mL of saline solution and fentanyl 0.02 mg/kg plus meloxicam 4 mg/kg at the incision site to alleviate postoperative pain and address any supplementary intraoperative fluid loss.</li>
	<li>Place the animal on a warmed surface to facilitate natural awakening, and monitor the blood supply to their extremities. Subsequently, house them individually in cages for continued observation as they regain consciousness.</li>
	<li>Subcutaneously inject medetomidine at a dose of 4 mg/kg upon recovery, continuing for 24 h postoperatively.</li>
</ol>
8. Intraoperative measurement of liver regeneration and liver function
<ol>
	<li>Euthanize the mice and collect liver as well as blood samples at intervals of 1, 2, 3, and 7 days post surgery.</li>
	<li>Perform HE staining and liver function test and examine serum inflammatory markers, thereby providing additional validation for the impact of the model construction.</li>
	<li>Document the daily variations in the body weight and FLR/BW (body weight) ratio of the mice over a span of 1 week following the surgical procedure.</li>
</ol>

Studying Liver Regeneration of ALLPS in Animal Models for Staged Hepatectomy

<ol>
	<li>Michalopoulos, G. K., Bhushan, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liver+regeneration:+Biological+and+pathological+mechanisms+and+implications.">Liver regeneration: Biological and pathological mechanisms and implications.</a> Nat Rev Gastroenterol Hepatol. 18 (1), 40-55 (2021).</li><li>Chapelle, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estimation+of+the+future+remnant+liver+function+is+a+better+tool+to+pr+edict+post-hepatectomy+liver+failure+than+platelet-based+liver+scores.">Estimation of the future remnant liver function is a better tool to pr edict post-hepatectomy liver failure than platelet-based liver scores.</a> Eur J Surg Oncol. 43 (12), 2277-2284 (2017).</li><li>Isfordink, C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Portal+vein+ligation+versus+portal+vein+embolization+for+induction+of+hypertrophy+of+the+future+liver+remnant:+A+systematic+review+and+meta-analysis.">Portal vein ligation versus portal vein embolization for induction of hypertrophy of the future liver remnant: A systematic review and meta-analysis.</a> Surg Oncol. 26 (3), 257-267 (2017).</li><li>Linecker, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Risk+adjustment+in+alpps+is+associated+with+a+dramatic+decrease+in+ear+ly+mortality+and+morbidity.">Risk adjustment in alpps is associated with a dramatic decrease in ear ly mortality and morbidity.</a> Ann Surg. 266 (5), 779-786 (2017).</li><li>Darnis, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subtotal+hepatectomy+in+swine+for+studying+small-for-size+syndrome+and+portal+inflow+modulation:+Is+it+reliable.">Subtotal hepatectomy in swine for studying small-for-size syndrome and portal inflow modulation: Is it reliable.</a> HPB (Oxford). 17 (10), 881-888 (2015).</li><li>Casellas, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inbred+mouse+strains+and+genetic+stability:+A+review.">Inbred mouse strains and genetic stability: A review.</a> Animal. 5 (1), 1-7 (2011).</li><li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolution+of+associating+liver+partition+and+portal+vein+ligation+for+staged+hepatectomy+from+2012+to+2021:+A+bibliometric+analysis.+Review.">Evolution of associating liver partition and portal vein ligation for staged hepatectomy from 2012 to 2021: A bibliometric analysis. Review.</a> Int J Surg. 103, 106648 (2022).</li><li>Parola, M., Pinzani, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liver+fibrosis:+Pathophysiology,+pathogenetic+targets+and+clinical+iss+ues.">Liver fibrosis: Pathophysiology, pathogenetic targets and clinical iss ues.</a> Mol Aspects Med. 65, 37-55 (2019).</li><li>Kikuchi, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+associating+liver+partition+and+portal+vein+occlusion+for+st+aged+hepatectomy+on+tumor+growth+in+a+mouse+model+of+liver+metastasis.">Impact of associating liver partition and portal vein occlusion for st aged hepatectomy on tumor growth in a mouse model of liver metastasis.</a> Eur J Surg Oncol. 44 (1), 130-138 (2018).</li><li>Schlegel, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alpps:+From+human+to+mice+highlighting+accelerated+and+novel+mechanism+s+of+liver+regeneration.">Alpps: From human to mice highlighting accelerated and novel mechanism s of liver regeneration.</a> Ann Surg. 260 (5), 839-846 (2014).</li><li>Dili, A., Lebrun, V., Bertrand, C., Leclercq, I. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Associating+liver+partition+and+portal+vein+ligation+for+staged+hepate+ctomy:+Establishment+of+an+animal+model+with+insufficient+liver+remnan+t.">Associating liver partition and portal vein ligation for staged hepate ctomy: Establishment of an animal model with insufficient liver remnan t.</a> Lab Invest. 99 (5), 698-707 (2019).</li><li>Schadde, E., Hertl, M., Breitenstein, S., Beck-Schimmer, B., Schl&#228;pfer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rat+model+of+the+associating+liver+partition+and+portal+vein+ligation+for+staged+hepatectomy+(alpps)+procedure.">Rat model of the associating liver partition and portal vein ligation for staged hepatectomy (alpps) procedure.</a> J Vis Exp. (126), e55895 (2017).</li><li>Allaire, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+frontiers+in+liver+resection+for+hepatocellular+carcinoma.">New frontiers in liver resection for hepatocellular carcinoma.</a> JHEP Rep. 2 (4), 100134 (2020).</li><li>Memi, F., Ntokou, A., Papangeli, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Crispr/cas9+gene-editing:+Research+technologies,+clinical+applications+and+ethical+considerations.">Crispr/cas9 gene-editing: Research technologies, clinical applications and ethical considerations.</a> Semin Perinatol. 42 (8), 487-500 (2018).</li><li>Saviano, A., Henderson, N. C., Baumert, T. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-cell+genomics+and+spatial+transcriptomics:+Discovery+of+novel+cell+states+and+cellular+interactions+in+liver+physiology+and+disease+biology.">Single-cell genomics and spatial transcriptomics: Discovery of novel cell states and cellular interactions in liver physiology and disease biology.</a> J Hepatol. 73 (5), 1219-1230 (2020).</li></ol>

The authors have no conflicts of interest to declare.

This study presents a protocol for the initial phase of ALPPS surgery in mice, involving partial ligation of the portal vein and division of the median liver lobe. This method closely mimics the human ALPPS procedure, with the mouse liver&#39;s unique lobular structure and dual portal venous supply to the median lobe ensuring distinct blood flow post-surgery11. It is noteworthy that the ALPPS procedure comprises two distinct surgical stages. In this study, we exclusively present the initial stage ...

The liver harbors formidable regenerative potential, swiftly proliferating and restoring metabolic demands within a mere 3 months following resection for diverse hepatic ailments1. However, the imperative to ascertain the completeness of tumor margins necessitates the inevitability of expansive hepatic excision. Thus, ensuring an ample volume of the participatory hepatic milieu, known as the future liver remnant (FLR), assumes paramount importance2. ALPPS has been a breakthrough technique in hepatic surgery over the past few decades, particularly tailored for patients with inadequate residual hepatic volume subsequent to tumor resection, heralded as one of the most auspicious breakthroughs in the realm of hepatic oncological surgery3.
Remarkable progress has been made in developing ALPPS animal models. An ideal model typically requires independent blood inflow (portal vein and hepatic artery) and outflow (hepatic vein) in the preserved hepatic lobe and a clear separation between the preserved and the to-be-resected hepatic lobes to prevent collateral circulation4. Although ALPPS stimulates rapid hepatic regeneration in the remaining liver tissue, the specific mechanisms of this process are still unclear.
Currently, ALPPS models are categorized into three types: large animal models (e.g., swine and sheep), medium-sized models (e.g., rabbits and rodents), and small models (e.g., mice)5. The use of mice, with their quick breeding and ease of genetic modification, is particularly effective for in-depth studies of hepatic regeneration mechanisms6. Moreover, the liver structure of mice, especially their middle hepatic vein, closely resembles that of humans, making them highly suitable for ALPPS model development.
It is important to note that most hepatocellular carcinoma patients in clinical practice have underlying liver conditions, unlike the healthy liver models typically used in studies7. Thus, using mice preconditioned with hepatic fibrosis or viral infections can more accurately simulate the surgical responses and postoperative liver regeneration seen in patients with various liver diseases8. This approach could reveal new therapeutic targets of clinical relevance.
So far, a few renowned research groups, such as those at the University of Zurich and the University of Tokyo, have successfully developed murine ALPPS models9,10. Creating a standardized murine model under controlled conditions could advance our understanding of the rapid liver regeneration observed after ALPPS procedures.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>5% glucose injection (500 mL)</td>
			<td>Shapuaisi pharma (http://www.zjspas.com/)</td>
			<td>H20003666</td>
			<td>Efficient, cheap,Easy to access</td>
		</tr>
		<tr>
			<td>anaesthesia machine</td>
			<td>RWD (www.rwdls.com)</td>
			<td>R500</td>
			<td>low price and valuable quality. It is suitable for operations with beginners</td>
		</tr>
		<tr>
			<td>C57 BL/6</td>
			<td>The Jackson Lab</td>
			<td>22349-2023</td>
			<td>Stability of strains</td>
		</tr>
		<tr>
			<td>isoflurane</td>
			<td>KCSW (kcsw.szqisoubao.com)</td>
			<td>H19980141</td>
			<td>durable, cost-effective</td>
		</tr>
		<tr>
			<td>meloxicam</td>
			<td>Boehringer-Ingelheim</td>
			<td>H20020217</td>
			<td>Durable and efficient</td>
		</tr>
		<tr>
			<td>microforceps</td>
			<td>maydeal</td>
			<td>60018920</td>
			<td>Durable and efficient</td>
		</tr>
		<tr>
			<td>microinstrument</td>
			<td>CH microsurgical instrument factory</td>
			<td>HC-A804-1</td>
			<td>durable, cost-effective</td>
		</tr>
		<tr>
			<td>sodium lactate ringer</td>
			<td>Shapuaisi pharma (http://www.zjspas.com/)</td>
			<td>H20193277</td>
			<td>Efficient, cheap, easy to access</td>
		</tr>
		<tr>
			<td>suture line</td>
			<td>CH microsurgical instrument factory</td>
			<td>6-0/8-0</td>
			<td>high performance-price ratio</td>
		</tr>
		<tr>
			<td>topical antibodies</td>
			<td>chenxin pharmacology (www.cisen-pharma.com)</td>
			<td>H20020217</td>
			<td>Effectively avoid incision infection</td>
		</tr>
	</tbody>
</table>

a mouse model of the associating liver partition and portal vein ligation for staged hepatectomy procedure aided by microscopy

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative mortality, severely impacting patient outcomes. In a robust hepatic environment, the future liver remnant (FLR) must exceed 25%, and in cases of cirrhosis, this requirement increases to over 40%. The inadequacy of FLR is currently a major obstacle in the progression of hepatic surgery.
Traditional methods to enhance FLR hypertrophy mainly focus on portal vein embolization (PVE), but its effectiveness is considerably limited. In recent years, there have been numerous reports on a novel biphasic hepatectomy method involving hepatic partitioning and portal vein ligation, known as associating liver partition and portal vein ligation for staged hepatectomy (ALPPS). ALPPS surpasses PVE in efficiently and considerably inducing FLR hypertrophy. However, the detailed mechanisms driving ALPPS-facilitated hepatic regeneration are not fully understood. Thus, replicating ALPPS in animal models is crucial to thoroughly investigate the molecular mechanisms of hepatic regeneration, offering valuable theoretical and practical insights.

Author Spotlight: Advancing Liver Regeneration Research through ALPPS Mouse Model

A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy

ALPPS can rapidly promote liver regeneration. However, the rather small animal model and the mechanism of the rhythm vision are still insufficient. This model allows for investigating the molecular and cellular pathway involved in liver regeneration of the severe liver injury With was the A of a ARVPS and the PMX mouse models, business studies showed that balance of PMX three and the round two in comparisons regulate haptic was killed under constitution, imposed operative liver regeneration.Single cell sequencing. Single cell attack sequencing. As well as single cell CRISPR has paved the way for the discovery of previously unknown cell types and subtypes in normal and list liver.The ARBs technique is used for patients with liver tumors that cannot be removed through surgery. The ability of the remaining liver to regenerate after the procedure is crucial for the patient's survival. However, the underlying mechanism post AFVBS remaining artery unknown.Based on the previous reports on the rat and the mouse AFVBS GECo models, we constructed a mouse GECo protocol and it was consistent with clinical operation and native limb.

In contrast to the PVL (protocol steps 2,3,4, and 5), the mice that were subjected to the ALPPS (steps 2,3,4,5, and 6) surgical intervention demonstrated a heightened proclivity towards cellular proliferation. Figure 2 delineates the ischemic demarcation of the middle liver lobe following portal vein ligation, along with the visual field during liver transection, which represents a key distinction between ALPPS and PVL. Notably, the levels of inflammatory mediators, including (Tumor Necrosis...

Read this Scientific Article Protocol about A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy at JoVE.com

Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) has been shown to result in remarkable liver regeneration after several weeks post first-stage surgery. Animal models that have undergone ALPPS have been developed to explore potential liver regeneration ability and achieve favorable clinical outcomes.

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative ...

a-mouse-model-associating-liver-partition-portal-vein-ligation-for

Research

JoVE Journal

Medicine

Associated Liver Partition and Portal Vein Ligation for Staged Hepatectomy for Studying Liver Regeneration in a Mouse Model

To begin, subcutaneously inject one milliliter of lactated Ringer's solution mixed with 5%glucose into the anesthetized mice. Use small animal clippers to shave a two-centimeter area on the abdominal region of the mouse. Then, make a midline incision with a surgical scalpel.Open the incision with a pair of surgical scissors. Use an abdominal retractor to expand the incision, while facilitating the gentle traction of the duodenum and a section of the small intestine with sterile, moist cotton swabs. Cover the structures with a pre-prepared sterile cotton swab to expose the hepatic segment of the portal vein.For portal vein ligation, peel off the peritoneum and intestinal tract. Encircle the main portal vein's branches with slow, deliberate, forward pushing and spreading movements. Identify the right posterior branch, the combined left lateral and median branches, and the caudate branch.Next, to dissect the right posterior branch, locate its prominent, visible branch when traversing the hepatic hilum via the main portal vein. Use moistened cotton balls as a fulling agent to separate the liver's middle lobe from the right posterior lobe. Dissect along the Glisson's capsule.Pull a 1.5-centimeter long silk tie around the portal vein branches. Then, use an 8-0 silk suture to ligate the right posterior branch. For the dissection of the left lateral and median branches, first, dissect any potential interstice between the left lateral lobe and the caudate lobe.Then, introduce a pair of micro forceps into the entrance created. When resistance is felt, adjust the micro forceps slightly to move through the surface of the Glisson's capsule of the right medial lobe. Tie an 8-0 silk suture around the portal vein of the left lateral and median branches.Successful ligation can be observed by the ischemic demarcation within the liver's middle lobe with pallor on the left lobe. Next, dissect the caudal lobe. Fill the potential space between the main portal vein and the caudate lobe with a cotton swab.Peel away the peritoneum of the caudate lobes to visualize the portal vein branches of the lobe. Ligate the caudate branch with a stitch-up suture, ensuring that the needle path adequately wraps the caudate branch. To transect the liver, use an electrocautery pen to create a 0.5-millimeter pre-cut line along the demarcation line in the middle liver lobe.With a pair of micro forceps and the electrocautery pen, cut the liver tissue. Swab the bleeding with cotton. Remove the gallbladder at the end, as it provides a good pulling point through transection.Use a cotton swab to apply controlled pressure for hemostasis. Once transection and cholecystectomy are complete, carefully place the small bowel back into the abdominal cavity. Close the peritoneum and the abdominal wall with 6-0 absorbable sutures.Disinfect the skin's surface, and then subcutaneously inject fentanyl and meloxicam mixed with saline to alleviate postoperative pain. Place the mouse on a thermostatic pad to facilitate awakening. Transfer them into individual cages when they regain consciousness.Subcutaneously inject medetomidine, continuing for 24 hours postoperatively. Mice that were subjected to ALPPS showed a higher proclivity to cellular proliferation. Inflammatory markers of the ALPPS mice, specifically interleukin-6, were higher than those observed in the PVL group.

331 Views.  The First Affiliated Hospital of Nanchang Medical College.  Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) has been shown to result in remarkable liver regeneration after several weeks post first-stage surgery. Animal models that have undergone ALPPS have been developed to explore potential liver regeneration ability and achieve favorable clinical outcomes.

Studying Liver Regeneration of ALLPS in Animal Models for Staged Hepatectomy

Summary